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Science 4 November 2005:
Vol. 310. no. 5749, pp. 855 - 857
DOI: 10.1126/science.1119052


Abstract
Full Text
Regulated Pole-to-Pole Oscillations of a Bacterial Gliding Motility Protein
Tâm Mignot, John P. Merlie Jr., David R. Zusman

Supporting Online Material

This supplement contains:

Materials and Methods
Table S1
SOM Text
Figs. S1 to S4
References
Movies S1 to S3

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  • Movie S1.
    FrzS dynamics in a reversing cell. Fluorescence microscopy time-lapse series of a FrzS-GFP expressing cell (10 min real time). We see relocalization of the brighter FrzS-GFP fluorescent patch from one cell pole to the other when the cell reverses.

  • Movie S2.
    FrzS dynamics in a hyper-reversing cell. Fluorescence microscopy time-lapse series of a FrzS-GFP frzCDc mutant cell (10 min real time). The cell pictured reversed its direction three times in the filming interval and showed corresponding oscillations in FrzS-GFP localization.

  • Movie S3.
    Moving FrzS cluster in a nonmoving cell. Fluorescence microscopy time-lapse series of a nonmoving cell (5 min real time) showing slow pole-to-pole movement of a FrzS cluster.

To view these movies, download a QuickTime viewer.





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Science. ISSN 0036-8075 (print), 1095-9203 (online)