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In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles
Benoit Dubertret, Paris Skourides, David J. Norris, Vincent Noireaux, Ali H. Brivanlou, and Albert Libchaber
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Supplementary Material
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This supplement contains:
Materials and Methods
References
Fig. S1:
QD resistance to photobleaching compared to GFP. (A) through (C)
are consecutive images of membrane-GFP expressed in Xenopus ectoderm (animal pole). (D) through (F) are consecutive images of QD-injected Xenopus animal pole blastomeres. (G) and (H) are graphs representing the GFP and QD fluorescence intensity respectively
through an 80min period during which both fluorophores were exposed to continuous excitation. (Bar = 30
m).
Movies S1, S2, S3
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- Movie 1
(qdbleaching(compressed).AVI) Time lapse imaging of QD injected ectodermal cells under constant excitation.
- Movie 2
(QD nuclear translocation 1(compressed).AVI) QD’s are cytoplasmic at early blastula stages but become nuclear (punctate staining) at laste blastula stages.
- Movie 3
(QD nuclear relocalization Short(compressed).AVI) Higher magnification movie of the nuclear translocation of the QD’s at late blastula
stages.
