Regulation of Mitochondrial Biogenesis in Skeletal Muscle by CaMK Hai Wu, Shane B. Kanatous, Frederick A. Thurmond, Teresa Gallardo, Eiji Isotani, Rhonda Bassel-Duby, and R. Sanders Williams

Supplementary Material

Supplemental Figure 1. Transgenic mice express CaMKIV* in skeletal muscle. (A) MCK-CaMKIV* transgene structure. A cDNA encoding a constitutively active form of CaMKIV (CaMKIV*) was inserted between a 4.8 kb MCK gene promoter and human growth hormone (hGH) poly-adenylation signal. (B) Northern blot analysis of transgene expression in different adult mouse tissues. Endogenous full-length CaMKIV was detected in brain. Calspermin, the testis-specific form of CaMKIV, lacks the C-terminal domain of CaMKIV and the transcript is shorter than the full-length CaMKIV (1). The constitutively active form of CaMKIV (CaMKIV*) was expressed only in skeletal muscles. Detection of -actin mRNA confirms RNA integrity.

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Supplemental Figure 2. The results of gene expression profiling by hybridization to custom microarrays that were generated from a murine skeletal muscle cDNA library representing 8,000 non-redundant clones. A list of eight genes that demonstrated greatest induction in plantaris muscles of CaMKIV* transgenic mice compared to wild type mice is shown.

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Reference

1. J. Y. Wu et al., Nature Genet. 25, 448 (2000).