Rap1 GTPase Regulation of Adherens Junction Positioning and Cell Adhesion Andrea L. Knox and Nicholas H. Brown

Supplementary Material

Supplemental Figure 1. Large clones of Rap1 mutant cells still respected the compartment boundary. The multiple wing hair-marked Rap1 clone in this wing had a growth advantage by being generated in a Minute background (by heat shockingy w P{ry^+t7.2 hsFLP}22; mwh Rap1^P[5709] P{w^hs FRT}2A/ M(3)i55 P{w^hs FRT}2A flies). It occupied the entire anterior compartment of the wing, and did not cross the compartment boundary that runs between veins L3 and L4. The dorsal edge of the clone is outlined in black and the ventral edge in red.

Medium version | Full size version

---

Supplemental Figure 2. Diagram of adherens junction distribution and cell movement in Rap1 mutant clones. Wild type cells are coloured green, and Rap1 cells white. Adherens junctions are represented by red lines, the width of the line corresponding to the amount present. In Rap1 cells, most adherens junctions are distributed to a contact with one other mutant cell. Normal levels are present at junctions between Rap1 and wild type cells. The Rap1 cell pairs separate from the body of the clone and move into surrounding wild type tissue because this allows them to form adherens junctions with all their neighboring cells.

Medium version | Full size version

---

Supplemental Figure 3. Time-lapse series showing transient concentration of GFP-Rap1 between sister cells in the developing embryo (arrowhead). Embryos expressing GFP-Rap1 were dechorionated by hand, mounted on a air-permeable teflon membrane in voltalef oil and covered with a coverslip. Images were captured with a MRC1024 confocal microscope (Bio-Rad). Bar 10m

Medium version | Full size version

---

Supplemental Figure 4. Model for how a requirement for Rap1 in sealing the adherens junction ring at cytokinesis could cause mislocalisation of adherens junctions to one side of Rap1 mutant cells.

Medium version | Full size version