An Apolipoprotein Influencing Triglycerides in Humans and Mice Revealed by Comparative Sequencing Len A. Pennacchio, Michael Olivier, Jaroslav A. Hubacek, Jonathan C. Cohen, David R. Cox, Jean-Charles Fruchart, Ronald M. Krauss, and Edward M. Rubin

Supplementary Material

Supplemental Figure 1. Profiles of lipoprotein particles separated by nondenaturing gradient-gel electrophoresis (GGE) from apoAV transgenic, knockout, and control mice. Lipoprotein particles (d 1.20 g/ml) were isolated by ultracentrifugation and subjected to GGE followed by lipid staining (1). (A) Comparison of lipoprotein particle profiles from knockout, heterozygous, and wild-type mice (mixed background strain C57BL6/129SvJ). The knockout data are the mean of two apoAV knockouts, whereas the heterozygote and control represent individual mice. (B) Comparison of the mean lipoprotein particle profile from apoAV transgenic and control littermates (n = 2 for each group) (isogenic strain FVBN).

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Reference

1. A. V. Nichols, R. M. Krauss, T. A. Musliner, Methods Enzymol. 128, 417 (1986).

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Supplemental Table 1. Association of the apoCIII Sst I RFLP with plasma triglycerides (mg/dl ± SEM). Human apoCIII Sst I polymorphism and triglyceride association data. Individuals (n = 501) were genotyped, and the number of successfully scored individuals is indicated. The minor allele frequency was 8.7%. Mutants: 1,1 is homozygous for the major allele; 1,2 is heterozygous for the major and minor alleles; 2,2 is homozygous for the minor allele.
Genotype	Sst I 1,1	Sst I 1,2	Sst I 2,2	P value (ANOVA)
n	320	61	7
Triglyceride
Random diet	121.1 ± 4.2	141.7 ± 10.7	139.1 ± 22.5	0.135
High-fat diet	101.1 ± 3.9	116.1 ± 8.6	124.0 ± 24.9	0.221
Low-fat diet	133.0 ± 6.3	155.1 ± 11.3	128.7 ± 18.5	0.345