Requirement of Tissue-Selective TBP-Associated Factor TAFII105 in Ovarian Development Richard N. Freiman, Shane R. Albright, Shuang Zheng, William C. Sha, Robert E. Hammer, and Robert Tjian

Medium version | Full size version

Supplemental Figure 2. Comprehensive results of microarray data indicating genes that were designated decreased or increased twofold or greater in the TAF_II105 mutant ovaries. Of over 13,000 probe sets tested on Affymetrix murine 11K arrays, only 309 genes fit such criteria. 132 genes are designated D or MD for decrease or marginally decrease, respectively. In addition, 177 genes are designated I or MI for increase or marginally increase, respectively. Probe sets represent Genbank accesion numbers or Msa numbers designated by Affymetrix. All data analysis was completed using microarray suite version 4.0 from Affymetrix.

Full size version

Supplemental Figure 3. Model of TAF_II105-mediated transcriptional activation in the ovary. Granulosa cell activator proteins (labeled G.C. Act.) expressed in granulosa cells of the developing follicle recruit TFIID to the core promoter of selective target genes. We postulate that this is accomplished through specific protein-protein interactions between activators (shown in yellow) and the TAF_II105 (shown in red) subunit of TFIID. Activation of the specific pathways and genes that have previously been implicated in folliculogenesis (shown by the arrows) requires TAF_II105 (see text). In the absence of TAF_II105, transcription of these select target genes is compromised leading to an arrest of folliculogenesis and female sterility in the TAF_II105-null mice.

Medium version | Full size version