Reciprocal Regulation Between TOC1 and LHY/CCA1 Within the Arabidopsis Circadian Clock David Alabadí, Tokitaka Oyama, Marcelo J. Yanovsky, Franklin G. Harmon, Paloma Más, and Steve A. Kay

Supplementary Material

Supplemental Figure 1. Extracts of E. coli expressing GST alone (lanes a, b), GST-CCA1 (lanes c, d), or GST-LHY (lanes e, f) at two different concentrations (1 in lanes a, c, e; and 2 in lanes b, d, f), were incubated for 20 minutes at room temperature with 1 ng of a 32P-labeled PCR product encompassing the -834 to -620 region of TOC1, and then analyzed by PAGE. The GST-CCA1 and GST-LHY protein concentration used in the competition experiments was 1 and 2, respectively. The arrow indicates the position of the protein-DNA complexes. FP, free probe. The black triangles indicate increasing extract concentration.

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