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Science 6 July 2001:
Vol. 293. no. 5527, pp. 95 - 97
DOI: 10.1126/science.1061402


Abstract
Full Text 		Epigenetic Instability in ES Cells and Cloned Mice
David Humpherys, Kevin Eggan, Hidenori Akutsu, Konrad Hochedlinger, William M. Rideout III, Detlev Biniszkiewicz, Ryuzo Yanagimachi, and Rudolf Jaenisch

Supplementary Material


Supplemental Figure 1. Southern analysis after digestion of placental DNA with Sac I and the methylation-sensitive restriction enzyme, Hha I. Blot was probed with H19 DMR Sac I fragment. Lanes contain DNA corresponding to samples in Fig. 1A, where lanes 1 through 5 are the same controls and lanes 6 through 14 match lanes 7 through 15, respectively.


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Supplemental Figure 2. Northern analysis comparison of H19 expression in placentas derived by the NT and tetraploid complementation techniques. Placentas from embryos that were in vitro cultured to the blastocyst stage (lanes 1 and 2) are shown for comparison.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)