SUB1, an Arabidopsis Ca2+-Binding Protein Involved in Cryptochrome and Phytochrome Coaction Hongwei Guo, Todd Mockler, Hien Duong, and Chentao Lin

Supplementary Material

Supplemental Figure 1. (A and B) Total RNA or protein extract of the wild-type (WT) or sub1 mutant (sub1) seedlings grown in white light (~100 mmol m^-2s^-1) were analyzed by an RNA blot probed with SUB1 or rRNA probe (A), or by an immunoblot probed with the antibodies against SUB1c (anti-SUB1c) or the polyclonal antibodies against pyrophosphatase (anti-PPase) for the loading control (B). (C) The hypocotyl length of the wild-type (WT), sub1 mutant (sub1), and two independent transgenic lines (35S-SUB1-2 and 35S-SUB1-14, both in the sub1 background) expressing the 35S::SUB1 transgene, were measured at day 5 after germination. The inserted immunoblot shows SUB1 protein level (and cry1 as a loading control) in different lines. Seedlings were grown in the dark (dark), blue light (blue, ~1 mmol m^-2s^-1), or far-red light (far-red, ~2 mmol m^-2s^-1) for 5 days. Hypocotyl lengths of 20 seedlings were measured for each sample, and standard deviations are shown.

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