Docosahexaenoic Acid, a Ligand for the Retinoid X Receptor in Mouse Brain Alexander Mata de Urquiza, Suya Liu, Maria Sjöberg, Rolf H. Zetterström, William Griffiths, Jan Sjövall, and Perlmann

Supplementary Material

Supplemental Figure 1. The RXR-activating factor(s) are enriched in the brain. (A) JEG-3 cells were transfected with UASx4-tk-luc reporter and GAL4-Nurr1 and RXR expression vectors, and then treated with media conditioned with indicated adult mouse tissues. Values are shown as fold induction after normalization to b-gal. The results show that media from testis and heart activated the reporter moderately while activation was very modest in media from spleen, kidney, and lung. Activation was most efficient in brain-conditioned medium. (B) JEG-3 cells transfected as in (A) were treated with conditioned media as depicted. Values are shown as fold induction after normalization to b-gal. The results suggest that RXR-activating factor(s) are present in several brain regions, showing that the activity is broadly distributed within the adult brain. Interestingly, however, the spinal cord apparently contains only low levels of RXR-inducing activity.

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