Manipulation of Host Hepatocytes by the Malaria Parasite for Delivery into Liver Sinusoids
Angelika Sturm, Rogerio Amino, Claudia van de Sand, Tommy Regen, Silke Retzlaff, Annika Rennenberg, Andreas Krueger, Jörg-Matthias Pollok, Robert Menard, Volker T. Heussler

Supporting Online Material

This supplement contains:
Materials and Methods
Figs. S1 to S8
Movies S1 to S4

This file is in Adobe Acrobat PDF format.

Supplementary Videos 1 to 4
Time-lapse intravital microscopy of a P. berghei-infected hepatocyte and budding vesicles (merosomes). Mice were infected with GFP-expressing P. berghei sporozoites and a liver lobe was exposed for microscopy at 51 hpi. Developing merosomes were followed up for 150 minutes. Video S1 shows parasite material (green) transported to a liver sinusoid labelled with red fluorescent BSA (red). S2 shows both, retraction of this parasite material to the mother cell and formation of a new merosome at a different place of the same cell. Video S3 and S4 show how this merosome increases with time by continous transfer of parasite material from the mother cell to the budding merosome. Time is individually indicated in minutes or seconds.

To view these movies, download a QuickTime viewer.