Arginylation of Beta Actin Regulates Actin Cytoskeleton and Cell Motility
Marina Karakozova, Marina Kozak, Catherine C. L. Wong, Aaron O. Bailey, John R.
Yates III, Alexander Mogilner, Henry Zebroski, Anna Kashina
Supporting Online Material
This supplement contains:
Materials and Methods
SOM Text
Figs. S1 to S5
Table S1
References
Videos S1 to S7
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Video S1
Time lapse images of the lamellar activity in a single wild-type mouse fibroblast. Stationary cell was chosen for continuous observation. Cell in the images undergoes global ruffling and contractility in the body, and forms a wide lamella along the cell edge.
Video S2
Time lapse images of the lamellar activity in a single Ate1–/– mouse fibroblast. Cell similar in morphology and behavior to the wild-type cell in Video 1 was chosen for comparison of lamella and ruffling activity.
Video S3
Time lapse images of the wild-type fibroblast monolayer moving into the wound. Moving cells form a wide lamella and cover the whole area of the substrate in the field of view over the time course of 8 hours.
Video S4
Time lapse images of the Ate1–/– fibroblast monolayer moving into the wound. Moving cells are unable to form a normal lamella and fail to efficiently cover the entire area of the substrate in the field of view over the time course of 8 hours.
Video S5
Time lapse images of an Ate1–/– fibroblast transfected with “permanently arginylated” beta actin. The cell forms a wide lamella during movement but continues to exhibit defects in ruffling activity and cortical flow characteristic for the Ate1–/– cells.
Video S6
Time lapse images of an Ate1–/– fibroblast transfected with “permanently arginylated” beta actin moving next to an untransfected Ate1–/– fibroblast for comparison. The transfected cell, unlike the untransfected one, forms a wide lamella during movement, indicating that the reintroduction of the arginylated beta actin restores the lamella formation phenotype in Ate1–/– cells.
Video S7
Time lapse images of an Ate1–/– fibroblast transfected with “permanently arginylated” beta actin moving within a monolayer of untransfected Ate1–/– cells during wound healing in culture. The transfected cell, unlike its multiple untransfected neighbors, forms a wide lamella during movement, indicating that the reintroduction of the arginylated beta actin restores the lamella formation phenotype in Ate1–/– cells.
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