Kinesin Walks Hand-Over-Hand Ahmet Yildiz, Michio Tomishige, Ronald D. Vale, Paul R. Selvin

This supplement contains:

Materials and Methods
Figs. S1 to S3
References and Notes

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• Movie S1: A single kinesin molecule moving on an immobilized axoneme.
  Movement of a dye on one head of a kinesin dimer along an immobilized axoneme. Cy3 fluorophore was attached to E215C and visualized using total internal reflection fluorescence microscopy. The movie was taken at 340 nM ATP concentration, 0.33 sec integration time per image. The pixel size was 86.6 nm. This spot lived 20 sec producing 60 images (Fig. S1A). The PSF fit well to 2-D Gaussian function revealing the steps of an individual head of kinesin dimer. Tracking of this spot is shown in blue trace in Fig. 2.