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Science 7 June 2002
DOI: 10.1126/science.1070051


Abstract
Full Text 		Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node
Mark J. Miller, Sindy H. Wei, Ian Parker, Michael D. Cahalan

Supplementary Material


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  • Movie 1
    A three-dimensional rotation showing the distribution of CFSE-labeled T cells (green) among reticular fibers stained with CMTMR (red). Room temperature.

  • Movie 2  size = 1,815 KB
    Time-lapse video showing changes in T lymphocyte motility as the temperature (in °C) was varied in sequence: 36, 28, 33, 36, 42. Inset shows temperature dependence of average velocities.

  • Movie 3
    Time-lapse montage of T cells in diffuse cortex and B cells in follicles.

  • Movie 4
    CFSE-labeled T cells (green) crawling among reticular fibers stained with CMTMR (red). Time-lapse images in the x-y (top) and x-z (bottom) planes.

  • Movie 5  size = 1,421 KB
    Changes in DO11.10 T cell motility that result from antigen activation and subsequent cell division. (A) Control, unprimed. (B) 1 day post-transfer into an ovalbumin-primed mouse, a stable cluster. (C) 1 day post-transfer into an ovalbumin-primed mouse, a swarming group of T cells. (D) 5 days post-transfer, dim and bright cells moving.

  • Movie 6
    Higher magnification of a stable cluster of DO11.10 T cells in the lymph node of ovalbumin-primed mouse, 1 day after transfer.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)