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Lack of Replicative Senescence in Normal Rodent Glia
Nicole F. Mathon, Denise S. Malcolm, Marie C. Harrisingh, Lili Cheng, Alison C. Lloyd
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Supplementary Material
Supplemental Figure 1. Representative fields of Schwann cells cultured in normal conditions (DMEM/3% FCS/1
mM forskolin/20 ng/ml GGF) (top left panel) and Schwann cells cultured in Schwann cell medium supplemented with 20% serum (other three panels). 4.8 × 10
5 cells early passage Schwann cells were seeded onto 90 mm dishes. Every three days cells were trypsinized and reseeded at constant density. These photographs were taken after 3 weeks continuous passaging. Antibodies used: Anti-cyclin D1-72-13G (Santa Cruz-450), anti-cyclin D2-M20 (Santa Cruz-182), anti-cyclin D3-C-16 (Santa Cruz-182), anti-cyclin E-M20 (Santa Cruz-481), anti-cyclin A-E23 (a gift from Julian Gannon ICRF, UK), anti-cdk-4-C-22 (Santa Cruz-260), anti-cdk-6 (Santa Cruz-177), anti-cdk-2-M2 (Santa Cruz-163), anti-p27Kip1-F8 (Santa Cruz-1641), anti-p21Cip1-C19 (Santa Cruz-397).

Medium version