Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 2 February 2001
DOI: 10.1126/science.1056782


Abstract
Full Text 		Lack of Replicative Senescence in Normal Rodent Glia
Nicole F. Mathon, Denise S. Malcolm, Marie C. Harrisingh, Lili Cheng, Alison C. Lloyd

Supplementary Material

Supplemental Figure 1. Representative fields of Schwann cells cultured in normal conditions (DMEM/3% FCS/1mM forskolin/20 ng/ml GGF) (top left panel) and Schwann cells cultured in Schwann cell medium supplemented with 20% serum (other three panels). 4.8 × 105 cells early passage Schwann cells were seeded onto 90 mm dishes. Every three days cells were trypsinized and reseeded at constant density. These photographs were taken after 3 weeks continuous passaging. Antibodies used: Anti-cyclin D1-72-13G (Santa Cruz-450), anti-cyclin D2-M20 (Santa Cruz-182), anti-cyclin D3-C-16 (Santa Cruz-182), anti-cyclin E-M20 (Santa Cruz-481), anti-cyclin A-E23 (a gift from Julian Gannon ICRF, UK), anti-cdk-4-C-22 (Santa Cruz-260), anti-cdk-6 (Santa Cruz-177), anti-cdk-2-M2 (Santa Cruz-163), anti-p27Kip1-F8 (Santa Cruz-1641), anti-p21Cip1-C19 (Santa Cruz-397).


Medium version

Return to article

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)