Fig. 3. Stat5 and Bcl6 regulation of menin in maternal islets. (A and B) Detection of Insulin (green) and Stat5 (red) in islets from nonpregnant (A) and pregnant (B) mice. Scale bar, 50 µm. (C) Western blot detection of Stat5, phospho-Stat5, and Bcl6 in extracts of maternal islets. (D) ChIP analysis of Bcl6 association with the Men1, p18, and p27 loci in islets isolated from nonpregnant, pregnant, postpartum day 4, and postpartum day 21 mice. Primer pair 2 (PP2) for Men1 has been described previously (7). (E and F) Real-time RT-PCR analysis of Men1, p18, and p27 (E) or Bcl6 mRNA levels (F) in islets exposed to prolactin (black), vehicle (white), or to both prolactin and progesterone (gray) (n = 3 independent experiments). Real-time RT-PCR analysis of Bcl6 (G) or Men1, p18, and p27 mRNA levels (H) in islets from prolactin-infused (black) and control mice (white). (I) Quantification of BrdU incorporation by islet ß-cells in prolactin-infused (black) or control mice (white) (n = 8 mice per treatment). *P < 0.05, **P < 0.01, ***P < 0.001. [View Larger Version of this Image (181K JPEG file)]

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