Fig. 2. Phenotypes from misexpression of menin in islets of pregnant mice. (A) Western blot analysis of the indicated proteins in islets from pregnant control and pregnant ßMen1 mice, both at 17 dpc, and from nonpregnant control mice. (B to E) Immunohistologic detection of nuclear menin [red, arrowheads in (D) and arrows in (E)] in islet ß-cells (green) from pregnant control (B and D) and pregnant ßMen1 (C and E) mice, both at 17 dpc. Scale bar, 50 µm (B and C) and 20 µm (D and E). (F) Blood glucose levels in ßMen1 mice (black) and controls (gray) fed ad libitum and exposed to Dox before, during, and after pregnancy (n = 10 to 15 mice per group). (G) Intraperitoneal glucose tolerance tests of Dox-exposed pregnant ßMen1 (black) and pregnant control mice (gray), both at 16 dpc (n = 3 to 6 mice per group). Calculated area under the curve was 25 ± 0.4 area units (ßMen1) versus 22 ± 0.7 area units (controls), P <0.005.(H) Serum insulin concentrations, (I) pancreatic ß-cell mass, and (J) BrdU incorporation studies in 16- to 17-dpc pregnant ßMen1 mice (black), pregnant controls (gray), and nonpregnant controls (white), all administered Dox (n = 3 to 6 mice per genotype). (K and L) Detection of BrdU (red, arrows) and insulin (green) in pregnant control (K) and pregnant ßMen1 mice (L) at 17 dpc. Scalebar, 50 µm. *P < 0.05, **P < 0.01, ***P < 0.001. [View Larger Version of this Image (184K JPEG file)]

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