Fig. 1. Dynamic regulation of menin and its target genes during facultative islet growth in pregnant mice. (A) Quantification of BrdU incorporation by maternal islet ß-cells (n = 3 to 6 mice sampled per time point). (B and C) Detection of insulin (green) and BrdU (red) in islets from nonpregnant (B) and 17-dpc pregnant mice (C). (D and E) Detection of insulin (green) and menin (red) in islets from nonpregnant (D) and 17-dpc pregnant female mice (E). Scale bar, 50 µm. (F and G) Real-time PCR analysis of Men1 and Mll (F) and Western blot analysis of the indicated proteins (G) from isolated maternal islets. (H) ChIP studies of menin and trimethylated H3K4 associated with p18 and p27 from nonpregnant, pregnant, and postpartum maternal islets (n = 3 to 6 mice). Data here and in Figs. 2, 3, 4 are presented as the means ± SD. *P < 0.05, **P < 0.01. Ab, antibody; IgG, immunoglobulin G; Menin, anti-menin Ab; Methyl, anti-H3K4 Ab; In, input DNA; IP, immunoprecipitate. [View Larger Version of this Image (178K JPEG file)]

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