Fig. 2. Localization of Ptc1 in primary cilia. (A) Concentration of endogenous Ptc1 in cilia of NIH 3T3 cells stimulated with 100 nM SAG. (B) Localization of Ptc1-YFP in ptc1^–/– MEFs infected with a retrovirus carrying an empty vector or the ptc1-YFP coding sequence. In (A) and (B), cilia (red) and Ptc1 (green) were visualized by immunofluorescence; nuclei (blue) were stained with DAPI. (C) Ptc1 staining in Shh-responsive cells of the neural tube (nt), notochord (nc), floor plate (fp), and paraxial mesoderm (m). Cross sections of wild-type (top row) or control ptc1^–/– (bottom row) mouse embryos (E9.5) were imaged with a 40x objective. (D) Asymmetric, ciliary localization of Ptc1 in paraxial mesoderm cells. The cell boxed in white is magnified in the bottom panel; arrows indicate Ptc1 staining (red) around the base and in the shaft of cilia (green). [View Larger Version of this Image (245K JPEG file)]

[PowerPoint download feature is available to paid individual subscribers and to registered users at subscribing institutions (register for free).]

You may download the image(s) above for non-profit educational presentation use only, provided no modifications are made to the content. Any use, publication, or distribution of the image(s) beyond that permitted in the sentence above or beyond that allowed by the "Fair Use" limitations (sections 107 and 108) of the US Copyright law requires the prior written permission of AAAS. This permission does not apply to images that are credited to non-AAAS sources. For images credited to non-AAAS entities, you will need to obtain permission from the entity listed in the legend or credit line before making any use of the image(s).

To Advertise   |   Find Products