Figure 2
Fig. 2. Innate immune receptor multiplicity in the sea urchin genome sequence. (A) Comparison of gene families encoding innate immune receptors in representative animals with sequenced genomes to S. purpuratus (bold, hereafter designated sea urchin). For some key receptor classes, gene numbers in the sea urchin exceed those of other animals by more than an order of magnitude. Representative animals are Homo sapiens, H.s.; C. intestinalis, C.i.; S. purpuratus, S.p.; D. melanogaster, D.m.; and C. elegans, C.e. Gene families include TLRs, NLRs, SRCRs, PGRPs, and GNBPs. Specifically, TLR diagrams show V, vertebrate-like, P, protostome-like; and S, short type; oval indicates TIR domain; and segmented partial circles indicate LRR regions; LRR-NT, blue; and LRR-CT, red. NLR diagram shows death family domain in pink, NACHT domain in yellow, and the LRR region, for which horizontal orientation implies cytoplasmic function. The other diagrams show multiple SRCR genes (both secreted and transmembrane), PGRP genes (PFAM: Amidase_2 domain–containing, secreted or transmembrane); and GNBP proteins (PFAM: Glyco_hydro_16–containing, secreted). For multiple SRCR genes, representative values are domain number (gene number in parentheses). For C. intestinalis, numbers correspond to all annotated SRCR proteins. Phylogenetic relations among species are indicated by the red cladogram at the left of the table; diagrams of molecules are not intended to imply specific structural features. (B) Unrooted neighbor-joining tree showing interrelations of TIR domains of TLRs in sea urchin. TLRs can be classified into three divergent classes (protostome-like, intron-containing, and short) and a large sea urchin lineage-specific family, which distributes into seven (I to VII) subgroups; numbers of member genes indicated in circles. Group I can be further subdivided [I(A) to I(E)]. Numbers beside branches indicate % bootstrap support for each subgroup. Efforts to relate vertebrate and other TLRs to the sea urchin genes result in low-confidence affinities with the divergent groups as described for other TLR comparisons (24). (C) Clustering of representative sea urchin TLR genes (yellow arrows) from high-confidence regions of the assembly supported by bacterial artificial chromosome (BAC) sequence (indicated by blue bar). Clusters segregate according to groups [I(B) and I(C) are subgroups of group I]. Gene model numbers are indicated above arrows. Model numbers with asterisks are close matches to annotated gene models and likely represent the second haplotype to that which was used to create models from the previous assembly. Red arrows indicate non-TLR genes. V indicates putative position of a V-type immunoglobulin domain cluster. Verification of cluster organization will require further independent genomic analysis. 
signifies pseudogene. Scale is indicated in kb (kilobase pairs).
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