Probing Gene Expression in Live Cells, One Protein Molecule at a Time
Ji Yu, Jie Xiao, Xiaojia Ren, Kaiqin Lao, X. Sunney Xie

Supporting Online Material

This supplement contains:
Materials and Methods
SOM Text
Figs. S1 to S6
Table S1
References
Movies S1 and S2

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MovieS1
Time-lapse movies (DIC/Fluorescence overlay) of SX4 cells under the repressing condition. Single fluorescent protein molecules are spontaneously generated, and clustered in gene expression bursts. The images were collected every 3 min with 100 ms acquisition time followed by 1100 ms photobleaching time. The total elapse time is 210 min for the first movie and 170 min for the second.

MovieS2
Time-lapse movies (DIC/Fluorescence overlay) of SX4 cells under the repressing condition. Single fluorescent protein molecules are spontaneously generated, and clustered in gene expression bursts. The images were collected every 3 min with 100 ms acquisition time followed by 1100 ms photobleaching time. The total elapse time is 210 min for the first movie and 170 min for the second.

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