Fig. 3. Loss of lin-14 function during adulthood is sufficient to extend life span. (A) Wild-type animals treated with lin-14(RNAi) (pink) only in the adult stage have extended life spans compared with mock RNAi animals (red). lin-4(e912)lf mutants treated with lin-14(RNAi) (light blue) only in the adult stage display an extended life span compared with lin-4(e912)lf mutants on mock RNAi (blue) at 20°C. N2: n = 55, m = 14.8. lin-14(RNAi): n = 55, m = 17.6, P < 0.0001*. lin-4(e912)lf: n = 43, m = 9.0. lin-4(e912)lf; lin-14(RNAi): n = 40, m = 12.1, P < 0.0001#. (B) lin-14(n179)lf animals (blue) display extended life spans compared with wild-type animals (red) when grown at the permissive temperature of 15°C until the larval-to-adult transition and then moved to the restrictive temperature of 25°C. N2: n = 48, m = 10.7. lin-14(n179)lf: n = 53, m = 12.4, P < 0.0001*. All experiments were repeated at least once with similar effects. P* and P# are defined in the legend to Fig. 1. [View Larger Version of this Image (128K JPEG file)]

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