Fig. 2. lin-4 and lin-14 mutants display accelerated and delayed rates, respectively, of lipofuscin accumulation. (A) lin-4(e912)lf mutants display an increase of lipofuscin as compared with similarly aged wild-type animals at 20°C. This effect is suppressed by the lin-14(n179)lf mutation. (B) Quantification of the N2, lin-4(e912)lf, lin-4(e912)lf;lin-14(n179)lf populations' gut autofluorescence at days 0, 4, 8, and 12 after the larval-to-adult transition at 20°C. (C) lin-14(n179)lf mutants display a decrease in lipofuscin accumulation compared with wild-type animals at 25°C. (D) Quantification of the N2 and lin-14(n179)lf populations' gut autofluorescence at 25°C as for (B). For (A) and (C), photographs shown are representative examples (n = 10 for each time point per strain). Photographs were taken at 100x magnification. All animals were photographed on the same day under identical conditions, and photographs were treated identically. For (B) and (D), autofluorescence was quantified using Axiovision 4.4 software (n = 10 for each time point per strain). P values were calculated using the Mann-Whitney nonparametric t test comparing mutant to wild-type results at day 12 in (B) and day 8 in (D). Error bars represent the standard error of the mean. [View Larger Version of this Image (176K JPEG file)]

[PowerPoint download feature is available to paid individual subscribers and to registered users at subscribing institutions (register for free).]

You may download the image(s) above for non-profit educational presentation use only, provided no modifications are made to the content. Any use, publication, or distribution of the image(s) beyond that permitted in the sentence above or beyond that allowed by the "Fair Use" limitations (sections 107 and 108) of the US Copyright law requires the prior written permission of AAAS. This permission does not apply to images that are credited to non-AAAS sources. For images credited to non-AAAS entities, you will need to obtain permission from the entity listed in the legend or credit line before making any use of the image(s).

To Advertise   |   Find Products