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A Magnetic Nanoprobe Technology for Detecting Molecular Interactions in Live Cells
Jaejoon Won, Mina Kim, Yong-Weon Yi, Young Ho Kim, Neoncheol Jung, Tae Kook Kim
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Supporting Online Material
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This supplement contains:
Materials and Methods
Figs. S1 to S6
Movie S1
References and Notes
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- Movie 1
Translocation of caspase3-mRFP by DEVD-coated superparamagnetic nanoparticles in
a magnetic field. HeLa cells were transfected with the expression plasmids for caspase3-mRFP or
EGFP, detached, mixed together, and replated. These cells were incubated with MNPs coated
with DEVD and TAT-HA2 in the presence of 100 µM chloroquine for 12 h. Confocal images
were taken in the absence or presence of a magnetic field. Cells expressing EGFP (green signals)
or caspase3-mRFP (red signals) were visualized by confocal microscopy with the focal plane
being at the basal surface of the cells. The free body diagram (designated as ?) indicates the
direction of the applied magnetic field, and the focal plane (cellular basal surface) can be
considered to be close to the viewer.
