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Salmonella Modulates Vesicular Traffic by Altering Phosphoinositide Metabolism
L. D. Hernandez, K. Hueffer, M. R. Wenk, J. E. Galán
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Supporting Online Material
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Materials and Methods
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- Movie S1
Time-lapse video microscopy of Henle-407 cells infected with either wild-type (left panel) or .sopB (right panel) strains. The entire sequence is 120 minutes from infection to the end of the experiment.
- Movie S2
Time-lapse video microscopy of Henle-407 cells expressing p40PX-YFP and infected with wild-type S. typhimurium expressing dsRed. Frames were taken every minute in the green and red channels.
- Movie S3
Time-lapse video microscopy of Henle-407 cells expressing p40PX-YFP and infected with S. typhimurium .sopB expressing dsRed. Frames were taken every minute. Left panel: green and red channels; right panel: phase.
- Movie S4
Time-lapse video microscopy of Cos-2 cells expressing p40PX-YFP and infected with wild-type S. typhimurium. Frames were taken every minute and the entire sequence, from infection to the end of the experiment is 90 minutes. Panel shows green channel.
- Movie S5
Time-lapse video microscopy of Cos-2 cells expressing p40PX-YFP and infected with wild-type S. typhimurium. Frames were taken every minute and the entire sequence, from infection to the end of the experiment is 90 minutes. Panel shows green channel.
- Movie S6
Time-lapse video microscopy of Henle-407 cells expressing p40PX-YFP and infected with wild-type S. typhimurium expressing dsRed. Frames were taken every minute. Left panel: green and red channels; middle panel: green channel only; right panel: phase.
- Movie S7
close up of the green and red channels of Movie S6.
- Movie S8
Time-lapse video microscopy of Henle-407 cells expressing p40PX-YFP and SopB-CFP. Frames were taken every minute and the entire sequence is 60 minutes. Left panel: phase; right panel: green channel.
- Movie S9
Time-lapse video microscopy of Ref52 cells expressing p40PX-YFP and SopB-CFP. Frames were taken every minute and the entire sequence is 60 minutes. Left panel: phase; right panel: green channel.
- Movie S10
Time-lapse video microscopy of Henel-407 cells expressing p40PX-YFP and SopE-CFP. Frames were taken every minute and the entire sequence is 60 minutes. Left panel: phase; right panel: green channel.