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Science 18 June 2004:
Vol. 304. no. 5678, pp. 1805 - 1807
DOI: 10.1126/science.1098188


Abstract
Full Text
Salmonella Modulates Vesicular Traffic by Altering Phosphoinositide Metabolism
L. D. Hernandez, K. Hueffer, M. R. Wenk, J. E. Galán

Supporting Online Material

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This supplement contains:

Materials and Methods
References

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To view these movies, download a QuickTime viewer.

  • Movie S1
    Time-lapse video microscopy of Henle-407 cells infected with either wild-type (left panel) or .sopB (right panel) strains. The entire sequence is 120 minutes from infection to the end of the experiment.

  • Movie S2
    Time-lapse video microscopy of Henle-407 cells expressing p40PX-YFP and infected with wild-type S. typhimurium expressing dsRed. Frames were taken every minute in the green and red channels.

  • Movie S3
    Time-lapse video microscopy of Henle-407 cells expressing p40PX-YFP and infected with S. typhimurium .sopB expressing dsRed. Frames were taken every minute. Left panel: green and red channels; right panel: phase.

  • Movie S4
    Time-lapse video microscopy of Cos-2 cells expressing p40PX-YFP and infected with wild-type S. typhimurium. Frames were taken every minute and the entire sequence, from infection to the end of the experiment is 90 minutes. Panel shows green channel.

  • Movie S5
    Time-lapse video microscopy of Cos-2 cells expressing p40PX-YFP and infected with wild-type S. typhimurium. Frames were taken every minute and the entire sequence, from infection to the end of the experiment is 90 minutes. Panel shows green channel.

  • Movie S6
    Time-lapse video microscopy of Henle-407 cells expressing p40PX-YFP and infected with wild-type S. typhimurium expressing dsRed. Frames were taken every minute. Left panel: green and red channels; middle panel: green channel only; right panel: phase.

  • Movie S7
    close up of the green and red channels of Movie S6.

  • Movie S8
    Time-lapse video microscopy of Henle-407 cells expressing p40PX-YFP and SopB-CFP. Frames were taken every minute and the entire sequence is 60 minutes. Left panel: phase; right panel: green channel.

  • Movie S9
    Time-lapse video microscopy of Ref52 cells expressing p40PX-YFP and SopB-CFP. Frames were taken every minute and the entire sequence is 60 minutes. Left panel: phase; right panel: green channel.

  • Movie S10
    Time-lapse video microscopy of Henel-407 cells expressing p40PX-YFP and SopE-CFP. Frames were taken every minute and the entire sequence is 60 minutes. Left panel: phase; right panel: green channel.





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Science. ISSN 0036-8075 (print), 1095-9203 (online)