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Cell Dynamics During Somite Boundary Formation Revealed by Time-Lapse Analysis
Paul M. Kulesa and Scott E. Fraser
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Supplementary Material
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To view these movies, download a QuickTime viewer.
- Movie 1: Cells widely disperse and frequently exchange neighbors near Hensen’s node.
Time-lapse series of DiI-labeled cell movements which follows cells from near the node until becoming allocated to a particular somite. The frames are 10 minutes apart
and the total duration is 16 hrs. Cell behaviors are summarized in Fig. 1.
- Movie 2: Within the region of segmentation, cells disperse less and maintain anteroposterior spacing.
Time-lapse series of DiI-labeled cells with images every 5 minutes for a total of 6 hrs. The cell behaviors are summarized in Fig. 1.
- Movie 3: Cell outlines show the local cell and tissue shapes in high-resolution within the region of somite separation from the segmental plate.
Ventral view of the somites with confocal sections (n=30) every 2 um. The cell and tissue shapes are summarized in Fig. 2.
- Movie 4: The somite pulls apart from the segmental plate.
Confocal time-lapse series of bodipy-ceramide labeled cells show the complex movements of cells across the presumptive somite boundary and separation of the forming somite from the segmental plate in a ball and socket shape. Each frame is 2 min. apart and the cell movements are summarized in Fig. 3.
- Movie 5: The somite pulls apart from the segmental plate.
This is the same movie as Movie 4 with subgroups of cells which cross the presumptive somite boundary marked by white and black dots.
