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Science 18 December 1998:
Vol. 282. no. 5397, pp. 2220 - 2226
DOI: 10.1126/science.282.5397.2220
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Research Articles

Structure of the MscL Homolog from Mycobacterium tuberculosis: A Gated Mechanosensitive Ion Channel

Geoffrey Chang, * Robert H. Spencer, * Allen T. Lee, Margaret T. Barclay, Douglas C. Rees dagger

Mechanosensitive ion channels play a critical role in transducing physical stresses at the cell membrane into an electrochemical response. The MscL family of large-conductance mechanosensitive channels is widely distributed among prokaryotes and may participate in the regulation of osmotic pressure changes within the cell. In an effort to better understand the structural basis for the function of these channels, the structure of the MscL homolog from Mycobacterium tuberculosis was determined by x-ray crystallography to 3.5 angstroms resolution. This channel is organized as a homopentamer, with each subunit containing two transmembrane alpha  helices and a third cytoplasmic alpha  helix. From the extracellular side, a water-filled opening approximately 18 angstroms in diameter leads into a pore lined with hydrophilic residues which narrows at the cytoplasmic side to an occluded hydrophobic apex that may act as the channel gate. This structure may serve as a model for other mechanosensitive channels, as well as the broader class of pentameric ligand-gated ion channels exemplified by the nicotinic acetylcholine receptor.

Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, 147-75CH, California Institute of Technology, Pasadena, CA 91125, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: dcrees{at}caltech.edu

The sensing of physical forces within a cell's environment is primarily mediated by a specialized class of membrane proteins known as mechanosensitive (MS) ion channels. MS channels have evolved the ability to transduce mechanical strain into an electrochemical response (1) enabling cells to respond to stimuli such as sound, touch, gravity, and pressure. Although several putative MS channels have been cloned, the large-conductance mechanosensitive channels (MscL) of prokaryotes have been most extensively characterized, primarily through the efforts of Kung and co-workers (2-4). MscL, first isolated from Escherichia coli, forms a nonselective ion channel of 2.5 nS conductance that is activated in vitro by the application of membrane tension. Localized within the inner membrane (5, 6), MscL is implicated in the regulation of osmotic pressure within the cell (7). MscL consists of a relatively small subunit (molecular mass of ~15 kD; Fig. 1) arranged as a homomultimer, possibly a hexamer, based upon cross-linking and electron microscopy studies (5, 6, 8). Hydropathy analysis indicates that MscL contains two transmembrane helices, and alkaline phosphatase fusion constructs suggest that both the NH2- and COOH-termini are colocalized within the cytoplasm (5). Following the identification of MscL in E. coli, homologs have been found in more than 20 divergent prokaryotic species (3, 9). Their primary sequences exhibit a strong degree of conservation, especially within the putative transmembrane regions (Fig. 1). Additionally, the mechanosensitive properties for many of these homologs have been confirmed by patch-clamp studies, although differences in gating kinetics and pressure sensitivity are evident (3).
Fig. 1. The amino acid sequence (40) alignment for several MscL homologs produced using the program CLUSTALX (41). Residues are colored according to their acidic (red), basic (blue), polar (green), or hydrophobic (gray) character. The transmembrane and cytoplasmic helices for the Tb-MscL structure are indicated above the sequence alignment for reference, and pore-lining residues are denoted by an asterisk, including residues facing into the cytoplasmic helical bundle. The sequence for M. leprae was identified in a BLAST search of data from the Sanger Genome Project and is included for comparison with the M. tuberculosis sequence (identity = 73%). The following GenBank accession numbers reference the sequences used for the alignments: B. subtilis, Z83337; E. coli, P23867; H. influenzae, U32745; M. tuberculosis, AL021999. [View Larger Version of this Image (51K GIF file)]

Structural characterization of MscL would establish not only the architecture of these channels but also aid in understanding the basic mechanism of mechanosensation. As recently demonstrated by the structure of the K+ channel from Streptomyces lividans (KcsA) (10), bacterial channels have important advantages for structural analyses, including relatively simple architecture and expressibility. We identified a MscL homolog in the pathogenic bacterium M. tuberculosis (Tb-MscL) that contains 151 amino acids and exhibits an overall 37% sequence identity to the E. coli MscL (Eco-MscL). The crystal structure of Tb-MscL determined at 3.5 Å resolution establishes the structural organization of this integral membrane protein and suggests possible gating mechanisms for opening and closing the channel in response to membrane stress.

Structure determination. Protein crystals are stabilized by interactions involving specific amino acids on the protein surface, so that variations in these surface residues can profoundly influence the formation of lattice contacts during crystallization. We therefore adopted the strategy of surveying crystallization space by cloning (11), expressing, and purifying (12) MscL homologs derived from nine prokaryotic species, with the expectation that one or more of these natural variants would be more optimal for crystal formation. Each of the full-length MscL ion channel constructs was cloned and recombinantly expressed in a mscL knockout strain of E. coli (2). Those channels and conditions that were found more suitable for protein purification and crystallization were pursued further. After screening and refining approximately 24,000 crystallization conditions for the nine MscL homologs, using ~20 detergents, we obtained several different crystal forms of MscL. The M. tuberculosis homolog yielded crystals of the best diffraction quality, and these were used for the x-ray structure determination (13).

Native crystals of Tb-MscL grew in space group I4122 (a = b = 212.96 Å, c = 96.43 Å) and diffracted to a resolution of ~7 Å using synchrotron radiation. Upon soaking these crystals in suitable heavy-atom compounds (Table 1), the space group unambiguously changed to I41 with similar unit cell dimensions (a = b = 212.42 Å, c = 98.05 Å). We believe that the unsoaked Tb-MscL crystals contained a distribution of disordered molecules and that the binding of these heavy atoms stabilized a unique orientation, resulting in the lower symmetry space group. In an effort to strengthen protein lattice contacts and improve the overall order within these crystals, an extensive series of detergents, small organic molecules, and heavy-metal compounds were screened as additives. One of these compounds, Na3Au(S2O3)2, significantly improved the x-ray diffraction quality of the Tb-MscL crystals, to a limiting resolution of 3.5 Å. This compound was later found to bind at a set of crystal lattice contacts (Fig. 2A). In view of the superior diffraction quality, data collected from this soaked crystal was used as the "native" data set for the structure determination. With diffraction data collected from several different heavy-metal soaked crystals, phases were determined by the methods of multiple isomorphous replacement and anomalous scattering using the package PHASES (14, 15). Initial electron density maps clearly revealed that the asymmetric unit contained a single pentamer, corresponding to a solvent content of ~85%. Iterative fivefold noncrystallographic averaging, solvent flattening, and phase extension yielded electron density maps of excellent quality for model building (Fig. 2B). A chemical model was built using the program CHAIN (16), and the protein sequence registration was established by the presence of appropriate density in the electron density maps for the aromatic groups in the transmembrane helices.

Table 1. Data collection and crystallographic analysis. All diffraction data used in the structural analysis were collected from single crystals at beamline 9-1 (lambda  = 0.98 Å) at the Stanford Synchrotron Radiation Laboratory (SSRL) except for the Xe data set, which was collected at beamline 7-1 (lambda  = 1.08 Å). Additional data sets were collected at beamline 5.0.2 at the Advanced Light Source (ALS) and also on laboratory x-ray sources (RAXIS II and RAXIS IV). All data sets were collected at -165°C and processed using the programs MOSFLM and SCALA (39). Because of their extremely fragile nature, the Tb-MscL crystals were stabilized with glutaraldehyde by vapor diffusing the crystals for at least 48 hours before heavy-atom soaking and data collection. In addition, the triethylene glycol concentration in the drop was gently increased to 38% by vapor diffusion for optimal cryo-protection for flash-cooling in liquid nitrogen or ethane. The incorporation and concentration of DDM in heavy-atom soaks was critical for maintaining the integrity and diffraction quality of the crystals.

Diffraction data
Na3Au(S2O3)2 (NH4)2WS4 (CH3)3Pb acetate Xe
Bragg spacing limits (Å) 20.0-3.5 20.0-3.5 20.0-7.0 20.0-7.0
Total observations 66,083 27,379 6,513 9,522
Unique observations 24,876 11,492 2,901 4,549
Rsym (%) 8.9 7.8 4.9 5.0
Completeness (%) 90 90 86 87

Fig. 2. Structure determination of Tb-MscL. (A) Packing diagram of Tb-MscL channels in the crystal. The space group is I41 and the unit cell is superimposed onto the crystal lattice. The yellow and green spheres indicate the positions of the bound Na3Au(S2O3)2 and (NH4)2WS4 compounds, respectively. The binding of Na3Au(S2O3)2 to the crystal packing sites significantly improved the diffraction resolution from ~7.0 to 3.5 Å. The (NH4)2WS4 bound within the cytoplasmic five-helix bundle of the channel. The inset represents the view from the extracellular side of the channel showing the relative positioning of the heavy atoms. (B) Stereoview of experimental electron density for the TM1 and TM2 transmembrane helices. The NH2-terminal end of the model (residue 10) is labeled. The density is contoured at 1sigma and rendered with BOBSCRIPT and RASTER-3D (42-44). [View Larger Version of this Image (62K GIF file)]

(continued).

Generation of experimental electron density
Phasing power
Na3Au(S2O3)2 2.0
(NH4)2WS4 2.3
Density correlation coefficient 45%
Overall figure of merit 0.56
Refinement statistics
Single model
R factor (20-3.5 Å) 40%
Rfree (20-3.5 Å) (10%) 42%
Nine models
R factor (20-3.5 Å) 26%
Rfree (20-3.5 Å) (10%) 35%
Average B factor 68 Å2
Model geometry
Bond length deviation 0.009 Å 
Bond angle deviation 1.6°

The Tb-MscL structure refinement was complicated by a rapid decrease in intensity of the diffraction pattern with resolution, corresponding to an overall temperature factor of ~100 Å2. Similar overall temperature factors were reported for the KcsA channel structure (10). The experimentally phased electron density maps, however, appeared to be of much better quality than would be expected for this temperature factor. This suggested that there was one predominant orientation for the channel in the crystal, likely stabilized by heavy-atom binding, with additional orientations that introduced a degree of positional disorder in the crystal. As a consequence, while the diffraction pattern remained relatively strong at lower resolution, the scattering contributions from this ensemble of protein and associated detergent interfered at higher resolution, resulting in a rapid decrease in intensities. Standard refinement algorithms are relatively poor at modeling positional disorder of this type (17) and, as a result, we were unable to refine any single model of Tb-MscL to values of R and Rfree below ~40 and ~42%, respectively. In an effort to model multiple orientations, we simultaneously refined nine copies of the structure against the native data (18-20) with very strict fivefold noncrystallographic harmonic constraints applied between monomers within a channel, using the program X-PLOR (21, 22). After molecular dynamics refinement, an ensemble of similar models (average rms deviation between Calpha atoms between models: <1.2 Å) was achieved with a crystallographic R factor of 26% and an Rfree of 35% against the native Tb-MscL data. Residue positions in the alpha  helical regions were well-defined in these models, whereas the loop regions were less well ordered. An averaged model with good stereochemistry was computed and used for structural analysis.

Structural organization of Tb-MscL. The pentameric arrangement observed crystallographically contrasts with earlier studies on Eco-MscL that had suggested a hexameric structure based on cross-linking experiments (5) and electron microscopy (8), although other studies were inconclusive on this point (6). Using the cross-linker disuccinimidyl suberate (DSS), we observed that cross-linked Tb-MscL and Eco-MscL proteins could each be separated by gel electrophoresis into a ladder of five bands, suggesting a pentameric association (Fig. 3). These results indicate that the organization of Tb-MscL observed in the crystal accurately represents the oligomeric state of the detergent-extracted protein.

Fig. 3. Cross-linking analysis of the oligomeric state of purified MscL protein. Aliquots of Tb-MscL (10 µg) or Eco-MscL (3 µg) purified in 0.1% DDM were subjected to cross-linking with the homobifunctional, water-insoluble reagent DSS (2 mM final concentration). Reactions were performed in phosphate-buffered saline (pH 7.5) for 5 min at 4°C and subsequently quenched with 100 mM Tris (pH 7.5). The protein samples were resolved using a 4 to 15% polyacrylamide gel and visualized using Coomassie brilliant blue. For unknown reasons, monomers of the purified MscL proteins migrate as double bands on the gel, as reported previously (2, 33). [View Larger Version of this Image (71K GIF file)]

The crystal structure establishes that Tb-MscL exists as a homopentamer composed of two domains, designated transmembrane and cytoplasmic. Both domains share the same fivefold axis relating subunits within the channel. Tb-MscL is ~85 Å in length, with the transmembrane and cytoplasmic domains accounting for 50 and 35 Å, respectively (Fig. 4). The diameters of the transmembrane and cytoplasmic domains are ~50 and 18 Å, respectively. The actual membrane-spanning region of Tb-MscL is ~35 Å in length and is primarily composed of hydrophobic residues in the transmembrane helices. As noted in other integral membrane proteins, there is a trend for aromatic residues to be localized near the membrane-aqueous phase interface (23, 24). In the case of Tb-MscL, aromatic residues predominate on the cytoplasmic side of the membrane, including two residues in TM1 (Phe27 and Phe34) and five in TM2 (Phe79, Phe80, Phe84, Tyr87, and Phe88).

Fig. 4. Structure of the homopentameric mechanosensitive channel from M. tuberculosis. Side (left) and top (extracellular; right) views of Tb-MscL were rendered using BOBSCRIPT and RASTER-3D (42-44). The monomers within the channel are individually colored. The NH2- and COOH-terminal ends of the cyan monomer are indicated, and the dimensions of the channel are shown. [View Larger Version of this Image (45K GIF file)]

Each subunit of Tb-MscL exhibits a relatively simple folding topology (Fig. 4). Starting from the NH2-terminus at the cytoplasmic side, the first transmembrane helix (TM1, residues 15 through 43) crosses the membrane and creates the bulk of the pore. TM1 is connected to the second transmembrane helix (TM2, residues 69 through 89) by a loop (residues 44 through 68) that dips into the pore region. TM2 returns to the cytoplasm along the outside of the channel, and is followed by a second loop (residues 90 through 101) that connects to the cytoplasmic helix (residues 102 through 115). In view of the net negative charge in this region (Fig. 5), it is possible that the cytoplasmic helix is stabilized in this structure by the low pH of the crystallization conditions. The remaining residues at the NH2- and COOH-termini are presumably disordered. The sequence positions of the two transmembrane helices roughly agree with the predictions of the hydropathy analysis (2, 5). Additionally, the polypeptide fold through the transmembrane domain is threaded in the opposite direction to that observed in the KcsA channel (10).

Fig. 5. (A) Molecular surface rendering of Tb-MscL viewed from the extracellular side of the membrane. The electrostatic potential was calculated using the program GRASP (45) assuming an ionic strength equivalent of 100 mM NaCl and a dielectric constant of 2 and 80 for protein and solvent, respectively. The surface coloring varies continuously from blue in more basic regions to red in more acidic areas. Note that the pore surface is highly polar and occluded at the cytoplasmic side of the channel. The deviation of the shading of the electrostatic potential surface from fivefold symmetry is a consequence of the off-axis placement of the light source used in this figure. (B) Cut-away side view of Tb-MscL to reveal the solvent-accessible surface within the channel. All atoms within a 50° wedge from the fivefold axis of the structure were removed for this representation. The yellow bars delineate the region of the hydrophobic surface where the lipid bilayer is presumed to be. The occluded region of the channel is indicated by the green arrows. [View Larger Version of this Image (41K GIF file)]

The helical axes for TM1 and TM2 within each subunit are tilted by ~28° with respect to the molecular fivefold axis, whereas the cytoplasmic helices are tilted by ~15°. Each TM1 helix contacts four surrounding helices (Fig. 4). These contacts involve the TM1 helices of the two adjacent subunits (crossing angle = -43°) and two TM2 helices: one within the same subunit (crossing angle = 135°) and the second from an adjacent subunit (crossing angle = 169°) (25). The TM2 helices of neighboring subunits do not directly contact each other, but rather are separated by ~20 Å. The most extensive set of interactions in the transmembrane domain occurs between the TM1 and TM2 helices of adjacent subunits, with a buried surface area of 2300 Å2. In contrast, the interactions between TM1 and TM2 helices within a subunit are less extensive and bury only 800 Å2 between residues 34 through 36 and 77 through 79. The cytoplasmic domain consists of helices packed together at a crossing angle of ~24° to form a helical bundle that buries ~1700 Å2 between adjacent monomers.

Pore structure. The pore of Tb-MscL appears to be extended along the fivefold axis through both the membrane and cytoplasmic domains. The radius of the transmembrane pore varies between ~2 to 18 Å and is partially occluded at the cytoplasmic surface (Fig. 5). In the transmembrane domain, the pore is lined with a series of hydrophilic residues from TM1, which include a series of four threonines (Thr25, Thr28, Thr32, and Thr35), a lysine (Lys33), and an aspartate (Asp36). Consequently, the interior surface of the channel has a distinctly polar character. Intriguingly, the abundance of threonine residues on this surface is reminiscent of the pore-forming M2 helix in the nicotinic acetylcholine receptor (26, 27). The hydrophobic residues Ile14 and Val21 create a constriction near the cytoplasmic surface at the NH2-terminal end of TM1. In view of the small diameter (~2 Å) of the channel in this region, it appears likely that the structure of the channel represents a closed, or nearly closed, state.

The loop region between the TM1 and TM2 helices creates a flap that forms the extracellular surface of the pore. Residues 58 through 64 are found deep in the pore of the channel and are less ordered, perhaps reflecting the flexibility of three glycine residues (Gly62, Gly63, and Gly64) at the bottom of the loop near Lys33 of TM1. Another interesting feature of the pore loop are two isoleucine residues (Ile59 and Ile61) which face two other isoleucine residues (Ile38 and Ile39) from the TM1 helix and may play some role in controlling the pore diameter.

Directly below the occluded region of the pore, there is a cluster of charged residues, including Arg98, Lys99, Lys100, Glu102, and Glu104. The binding of oxyanionic heavy atoms from several different derivatives within this region of the cytoplasmic domain suggests a possible ligand-binding site, and the accessibility of this region could further imply that this part of the structure could potentially participate in part of the ion conduction pathway. In particular, a Xe-pressurized crystal (28) shows a tube of difference electron density along the fivefold axis of the cytoplasmic bundle (29). The juxtaposition of the transmembrane and cytoplasmic domains with coincident molecular axes, including the possible extension of the transmembrane channel through the cytoplasmic domain, resembles the proposed model for the interaction of the cytoplasmic NH2-terminal domain and channel regions of the Shaker K+ channel (30).

Relationship of structure to function. Unlike the cloned ligand- and voltage-gated ion channels that have been extensively characterized by a combination of electrophysiological and molecular biological techniques, the first mechanosensitive ion channel (Eco-MscL) was cloned relatively recently (2), and thus more limited information is currently available to relate MscL function to the structural model. However, mutagenesis studies with Eco-MscL have resulted in the mapping of several regions involved in the gating and pressure sensitivity of MscL (31-33). Considering the sequence similarity of Tb-MscL to Eco-MscL, these data yield significant insights into the domains responsible for MscL function.

The TM1 helix is one of the mostly highly conserved regions among MscL proteins, particularly at the NH2-terminal end (3). Single-site mutagenesis of Eco-MscL along this region showed that mutations along one face of this helix significantly alter channel gating (31, 32). Based upon the Tb-MscL model, these mutations consistently map to residues facing the ion conduction pathway. Mutants that display a gain-of-function phenotype have the most profound effects at positions 22, 23, 26, and 30 of Eco-MscL (equivalent to positions 20, 21, 24, and 28 of Tb-MscL). It is believed that these mutants result in channels that readily open and thus inhibit growth due the loss of ions out of the cell (31).

Because studies of the purified Eco-MscL channel reconstituted into lipid bilayers demonstrated that gating is intrinsic to this protein (2, 34), some integral structural rearrangement must occur to coordinate channel opening. Based upon the Tb-MscL structure, it would appear that the junction of the TM1 helices near the base of the channel can form a physical hydrophobic barrier or "gate" that occludes the channel, thereby restricting the permeation of ions through the pore. More significantly, several of the most severe gain-of-function mutants map within this hydrophobic apex (31), which supports the hypothesis that this region acts as the channel gate. Presumably, upon applying lateral tension to the membrane, rearrangement of the transmembrane region occurs such that the TM1 helices are pulled away from each other (Fig. 6), perhaps inserting between TM2 helices at the outer edge of the channel. Adjacent TM2 helices are separated by ~20 Å and, hence, could accommodate a repositioned TM1 helix (diameter of ~10 Å) between them. As a result of this proposed movement of the TM1 helices, the constriction of the central pore would be relaxed, thus allowing ions to cross the membrane. It has been reported that the cross-sectional area in the membrane occupied by MscL increases by ~350 Å2 between the closed and open states of the channel (4, 35). Although the structure of the open state of Tb-MscL has yet to be determined, a change of this magnitude could be achieved upon channel opening by a relatively modest increase in the effective diameter of the transmembrane domain from ~50 to ~51 Å.

Fig. 6. Proposed model for the mechanosensitive gating of Tb-MscL. Side (left) and top (extracellular; right) views of the Tb-MscL channel with arrows indicating the proposed shift in relative helical position upon application of tension to the membrane. To provide accessibility to the ion conduction pathway, the position of the TM1 helices near the cytoplasm would need to shift radially from the molecular fivefold axis (normal to the membrane). Possible movement of the TM2 and cytoplasmic helices are also indicated. This figure was prepared using InsightII molecular modeling system (MSI, San Diego, CA). [View Larger Version of this Image (37K GIF file)]

Pentameric organizations have been previously described for the important class of ligand-gated ion channels that include the nicotinic acetylcholine (AChR), serotonin (5HT3), GABAA, and glycine receptors (36). Beyond their common pentameric organization, the pore architecture of Tb-MscL exhibits strong similarities to that observed for the AChR (37, 38). In particular, the orientation, helix packing, and tilt angle of the M2 helices that have been proposed as forming the AChR pore correspond to those observed for the pore helices in Tb-MscL. The pore helices for both families appear to be lined with polar residues such as threonine or serine, and hydrophobic residues are present at the constriction in the pore that may act as the channel gate. The gating mechanism proposed for AChR involves a reorientation of the M2 helices that repositions the occluding hydrophobic residue, thereby leading to an open channel configuration. Although the detailed gating mechanisms undoubtedly differ between MscL and AChR, it seems quite plausible that they are both based on rearrangements of the pore helices that ultimately regulate the channel dimensions.

The structure of Tb-MscL provides a framework for understanding the transduction of mechanical stress on the lipid bilayer into protein conformational changes that control ion flux through the cell membrane. As a member of a family of mechanosensitive channels that are prevalent in bacteria, the structure of Tb-MscL should serve as a model for these related MscL channels and may additionally help to address aspects of the functional mechanism of nonhomologous, two-transmembrane-helix MS channels found in eukaryotes. Structural similarities in the pore regions between Tb-MscL and the family of pentameric ligand-gated ion channels also suggest that this structure will be useful for modeling gating mechanisms in a broad class of gated channels. As originally demonstrated with the structure of the bacterial photosynthetic reaction center (23), and most recently with the KcsA channel structure (10) and our work here, bacterial membrane proteins provide attractive systems for structural and functional characterization of more complex homologs of integral membrane proteins found in eukaryotes.

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29 October 1998; accepted 16 November 1998


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Am J Physiol Lung Cell Mol Physiol 277, L667-L683
   Abstract »    Full Text »    PDF »
Molecular Identification of a Eukaryotic, Stretch-Activated Nonselective Cation Channel.
M. Kanzaki, M. Nagasawa, I. Kojima, C. Sato, K. Naruse, M. Sokabe, and H. Iida (1999)
Science 285, 882-886
   Abstract »    Full Text »
Structural Rearrangements Underlying K+-Channel Activation Gating.
E. Perozo, D. Marien, Cortes, and L. G. Cuello (1999)
Science 285, 73-78
   Abstract »    Full Text »
Mechanosensitive channels in bacteria as membrane tension reporters.
S. SUKHAREV (1999)
FASEB J 13, 55-61
   Abstract »    Full Text »    PDF »
TREK-2, a New Member of the Mechanosensitive Tandem-pore K+ Channel Family.
H. Bang, Y. Kim, and D. Kim (2000)
J. Biol. Chem. 275, 17412-17419
   Abstract »    Full Text »    PDF »
Correlating a Protein Structure with Function of a Bacterial Mechanosensitive Channel.
P. C. Moe, G. Levin, and P. Blount (2000)
J. Biol. Chem. 275, 31121-31127
   Abstract »    Full Text »    PDF »
Comparing and Contrasting Escherichia coli and Mycobacterium tuberculosis Mechanosensitive Channels (MscL). NEW GAIN OF FUNCTION MUTATIONS IN THE LOOP REGION.
J. A. Maurer, D. E. Elmore, H. A. Lester, and D. A. Dougherty (2000)
J. Biol. Chem. 275, 22238-22244
   Abstract »    Full Text »    PDF »
Gramicidin A channels switch between stretch activation and stretch inactivation depending on bilayer thickness.
B. Martinac and O. P. Hamill (2002)
PNAS 99, 4308-4312
   Abstract »    Full Text »    PDF »
Conformational pathways in the gating of Escherichia coli mechanosensitive channel.
Y. Kong, Y. Shen, T. E. Warth, and J. Ma (2002)
PNAS 99, 5999-6004
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)