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Science 7 April 2006:
Vol. 312. no. 5770, p. 55
DOI: 10.1126/science.1115362
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Technical Comments

Response to Comment on "PDK1 Nucleates T Cell Receptor-Induced Signaling Complex for NF-{kappa}B Activation"

Ki-young Lee, Jae-Hyuck Shim, Matthew S. Hayden, Jan-Schulze Luehrmann and Sankar Ghosh*

Section of Immunology and Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA.


Figure 1 Fig. 1. Jurkat cells were stimulated as described previously and after centrifugation were lysed directly with radioimmunoprecipitation assay (RIPA) buffer (containing protease and phosphatase inhibitors) for 15 min on ice. After addition of SDS-loading buffer, equal amounts of the samples were fractionated on SDS–polyacrylamide gel electrophoresis, followed by transfer to polyvinylidene fluoride (PVDF) membranes. Immunoblotting of the membrane was carried out using standard protocols (BD Pharmingen). [View Larger Version of this Image (51K GIF file)]
 

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Science. ISSN 0036-8075 (print), 1095-9203 (online)