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Serine-7 of the RNA Polymerase II CTD Is Specifically Required for snRNA Gene Expression
Sylvain Egloff,1Dawn O'Reilly,1Rob D. Chapman,2Alice Taylor,1Katrin Tanzhaus,1Laura Pitts,1Dirk Eick,2Shona Murphy1*
RNA polymerase II (Pol II) transcribes genes that encode proteinsand noncoding small nuclear RNAs (snRNAs). The carboxyl-terminalrepeat domain (CTD) of the largest subunit of mammalian RNAPol II, comprising tandem repeats of the heptapeptide consensusTyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7, is required for expressionof both gene types. We show that mutation of serine-7 to alaninecauses a specific defect in snRNA gene expression. We also presentevidence that phosphorylation of serine-7 facilitates interactionwith the snRNA gene–specific Integrator complex. Thesefindings assign a biological function to this amino acid andhighlight a gene type–specific requirement for a residuewithin the CTD heptapeptide, supporting the existence of a CTDcode.
1 Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK. 2 Institute of Clinical Molecular Biology and Tumour Genetics, Gesellschaft fuer Strahlung Forschung (GSF)-Research Centre of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany.
* To whom correspondence should be addressed. E-mail: shona.murphy{at}path.ox.ac.uk
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PERSPECTIVES
Jeffry L. Corden (14 December 2007) Science318 (5857), 1735.
[DOI: 10.1126/science.1152624] |Summary »|Full Text »|PDF »
REPORTS
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[DOI: 10.1126/science.1145977] |Abstract »|Full Text »|PDF »|Supporting Online Material »
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