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Science 3 August 2007:
Vol. 317. no. 5838, pp. 660 - 663
DOI: 10.1126/science.1139952
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Reports

Quantitative Mass Spectrometry Identifies Insulin Signaling Targets in C. elegans

Meng-Qiu Dong,1 John D. Venable,1 Nora Au,2,3 Tao Xu,1 Sung Kyu Park,1 Daniel Cociorva,1 Jeffrey R. Johnson,1 Andrew Dillin,2 John R. Yates, III1*

DAF-2, an insulin receptor–like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.

1 Scripps Research Institute, La Jolla, CA92037, USA.
2 Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
3 University of California at San Diego, La Jolla, CA 92093, USA.

* To whom correspondence should be addressed. E-mail: jyates{at}scripps.edu

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Proteomics in Caenorhabditis elegans.
A. Audhya and A. Desai (2008)
Brief Funct Genomic Proteomic 7, 205-210
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The Caenorhabditis elegans K10C2.4 Gene Encodes a Member of the Fumarylacetoacetate Hydrolase Family: A CAENORHABDITIS ELEGANS MODEL OF TYPE I TYROSINEMIA.
A. L. Fisher, K. E. Page, G. J. Lithgow, and L. Nash (2008)
J. Biol. Chem. 283, 9127-9135
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Distinct Activities of the Germline and Somatic Reproductive Tissues in the Regulation of Caenorhabditis elegans' Longevity.
T. M. Yamawaki, N. Arantes-Oliveira, J. R. Berman, P. Zhang, and C. Kenyon (2008)
Genetics 178, 513-526
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Science. ISSN 0036-8075 (print), 1095-9203 (online)