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Science 27 April 2007:
Vol. 316. no. 5824, pp. 585 - 587
DOI: 10.1126/science.1138751
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Reports

A Dinuclear Ni(µ-H)Ru Complex Derived from H2

Seiji Ogo,1* Ryota Kabe,1,2 Keiji Uehara,2 Bunsho Kure,1,2 Takashi Nishimura,2 Saija C. Menon,2 Ryosuke Harada,1 Shunichi Fukuzumi,2 Yoshiki Higuchi,3 Takashi Ohhara,4 Taro Tamada,4 Ryota Kuroki4

Models of the active site in [NiFe]hydrogenase enzymes have proven challenging to prepare. We isolated a paramagnetic dinuclear nickel-ruthenium complex with a bridging hydrido ligand from the heterolytic cleavage of H2 by a dinuclear NiRu aqua complex in water under ambient conditions (20°C and 1 atmosphere pressure). The structure of the hexacoordinate Ni(µ-H)Ru complex was unequivocally determined by neutron diffraction analysis, and it comes closest to an effective analog for the core structure of the proposed active form of the enzyme.

1 Center for Future Chemistry, Kyushu University, Fukuoka 819–0395, Japan.
2 Department of Material and Life Science, Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, Solution-Oriented Research for Science and Technology, Japan Science and Technology Agency (JST), Suita, Osaka 565–0871, Japan.
3 Department of Life Science, Graduate School of Life Science, University of Hyogo, Koto, Kamigori, Hyogo 678–1297, Japan.
4 Research Group for Molecular Structural Biology, Quantum Beam Science Directorate, Japan Atomic Energy Agency, Tokai, Ibaraki 319–1195, Japan.

* To whom correspondence should be addressed. E-mail: ogo-tcm{at}mbox.nc.kyushu-u.ac.jp

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Crystal Structure of [Fe]-Hydrogenase Reveals the Geometry of the Active Site.
S. Shima, O. Pilak, S. Vogt, M. Schick, M. S. Stagni, W. Meyer-Klaucke, E. Warkentin, R. K. Thauer, and U. Ermler (2008)
Science 321, 572-575
   Abstract »    Full Text »    PDF »
Thiolate-bridged dinuclear iron(tris-carbonyl)-nickel complexes relevant to the active site of [NiFe] hydrogenase.
Y. Ohki, K. Yasumura, K. Kuge, S. Tanino, M. Ando, Z. Li, and K. Tatsumi (2008)
PNAS 105, 7652-7657
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)