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Science 20 October 2006:
Vol. 314. no. 5798, pp. 446 - 449
DOI: 10.1126/science.1131847
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Reports

Molecular Imaging Using a Targeted Magnetic Resonance Hyperpolarized Biosensor

Leif Schröder,1,2 Thomas J. Lowery,1,3 Christian Hilty,1,2 David E. Wemmer,1,3* Alexander Pines1,2*

A magnetic resonance approach is presented that enables high-sensitivity, high-contrast molecular imaging by exploiting xenon biosensors. These sensors link xenon atoms to specific biomolecular targets, coupling the high sensitivity of hyperpolarized nuclei with the specificity of biochemical interactions. We demonstrated spatial resolution of a specific target protein in vitro at micromolar concentration, with a readout scheme that reduces the required acquisition time by >3300-fold relative to direct detection. This technique uses the signal of free hyperpolarized xenon to dramatically amplify the sensor signal via chemical exchange saturation transfer (CEST). Because it is ~10,000 times more sensitive than previous CEST methods and other molecular magnetic resonance imaging techniques, it marks a critical step toward the application of xenon biosensors as selective contrast agents in biomedical applications.

1 Department of Chemistry, University of California, Berkeley, CA 94720, USA.
2 Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
3 Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

* To whom correspondence should be addressed. E-mail: dewemmer{at}lbl.gov (D.E.W.); pines{at}berkeley.edu (A.P.)

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Science. ISSN 0036-8075 (print), 1095-9203 (online)