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This article has been retracted

Science 1 July 2005:
Vol. 309. no. 5731, pp. 121 - 125
DOI: 10.1126/science.1112869
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Reports

A Magnetic Nanoprobe Technology for Detecting Molecular Interactions in Live Cells

Jaejoon Won,1 Mina Kim,1 Yong-Weon Yi,1 Young Ho Kim,2 Neoncheol Jung,2 Tae Kook Kim1*

Technologies to assess the molecular targets of biomolecules in living cells are lacking. We have developed a technology called magnetism-based interaction capture (MAGIC) that identifies molecular targets on the basis of induced movement of superparamagnetic nanoparticles inside living cells. Efficient intracellular uptake of superparamagnetic nanoparticles (coated with a small molecule of interest) was mediated by a transducible fusogenic peptide. These nanoprobes captured the small molecule's labeled target protein and were translocated in a direction specified by the magnetic field. Use of MAGIC in genome-wide expression screening identified multiple protein targets of a drug. MAGIC was also used to monitor signal-dependent modification and multiple interactions of proteins.

1 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
2 CGK Co. Ltd., Daejeon 305-701, Korea.

* To whom correspondence should be addressed. E-mail: tkkim{at}kaist.ac.kr

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Retraction.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)