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Science 25 July 1980:
Vol. 209. no. 4455, pp. 497 - 499
DOI: 10.1126/science.6248960

Articles

Science, Vol 209, Issue 4455, 497-499
Copyright © 1980 by American Association for the Advancement of Science


articles

Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen

BB Knowles, CC Howe, and DP Aden

Analysis of the cell culture fluid from two new human hepatoma-derived cell lines reveals that 17 of the major human plasma proteins are synthesized and secreted by these cells. One of these cell lines, Hep 3B, also produces the two major polypeptides of the hepatitis B virus surface antigen. When Hep 3B in injected into athymic mice, metastatic hepatocellular carcinomas appear. These cell lines provide experimental models for investigation of plasma protein biosynthesis and the relation of the hepatitis B viru genome to tumorigenicity.


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Y. Hayashi, Y. Kitamura, M. Nakanishi, and K. Koike (2000)
J. Virol. 74, 9471-9478
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Identification of an enhancer and an alternative promoter in the first intron of the {alpha}-fetoprotein gene.
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Cytotoxicity Assessment of Ma-huang (Ephedra) under Different Conditions of Preparation.
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Endocrinology 141, 2361-2369
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The Hepatitis B Virus Core Promoter Is Strongly Activated by the Liver Nuclear Receptor Fetoprotein Transcription Factor or by Ectopically Expressed Steroidogenic Factor 1.
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Antiproliferative and apoptotic effects of O-Trensox, a new synthetic iron chelator, on differentiated human hepatoma cell lines.
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J. Biol. Chem. 275, 5867-5873
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P. Kienle, J. Weitz, R. Klaes, M. Koch, A. Benner, T. Lehnert, C. Herfarth, and M. von Knebel Doeberitz (2000)
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Real time imaging reveals a peroxisomal reticulum in living cells.
M Schrader, S. King, T. Stroh, and T. Schroer (2000)
J. Cell Sci. 113, 3663-3671
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An 80-Kilodalton Protein That Binds to the Pre-S1 Domain of Hepatitis B Virus.
C. J. Ryu, D.-Y. Cho, P. Gripon, H. S. Kim, C. Guguen-Guillouzo, and H. J. Hong (2000)
J. Virol. 74, 110-116
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Requirement for Stat5 in Thymic Stromal Lymphopoietin-Mediated Signal Transduction.
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J. Immunol. 163, 5971-5977
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Hepatitis C virus and other Flaviviridae viruses enter cells via low density lipoprotein receptor.
V. Agnello, G. Abel, M. Elfahal, G. B. Knight, and Q.-X. Zhang (1999)
PNAS 96, 12766-12771
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An Autoregulatory Loop Controlling CYP1A1 Gene Expression: Role of H2O2 and NFI.
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Production of Thrombopoietin by Human Carcinomas and Its Novel Isoforms.
Y. Sasaki, T. Takahashi, H. Miyazaki, A. Matsumoto, T. Kato, K. Nakamura, S. Iho, Y. Okuno, and K. Nakao (1999)
Blood 94, 1952-1960
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Induction of Tubular Peroxisomes by UV Irradiation and Reactive Oxygen Species in HepG2 Cells.
M. Schrader, R. Wodopia, and H. D. Fahimi (1999)
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Use of the Hepatitis B Virus Recombinant Baculovirus-HepG2 System to Study the Effects of (-)-beta -2',3'-Dideoxy-3'-Thiacytidine on Replication of Hepatitis B Virus and Accumulation of Covalently Closed Circular DNA.
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Dual Signaling Role of the Protein Tyrosine Phosphatase SHP-2 in Regulating Expression of Acute-Phase Plasma Proteins by Interleukin-6 Cytokine Receptors in Hepatic Cells.
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Formation of a primitive ectoderm like cell population, EPL cells, from ES cells in response to biologically derived factors.
J Rathjen, J. Lake, M. Bettess, J. Washington, G Chapman, and P. Rathjen (1999)
J. Cell Sci. 112, 601-612
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Regulated turnover of a cell surface-associated pool of newly synthesized apolipoprotein E in HepG2 cells.
M. Schmitt and T. Grand-Perret (1999)
J. Lipid Res. 40, 39-49
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Modulation of Human Mineralocorticoid Receptor Function by Protein Kinase A.
C. Massaad, N. Houard, M. Lombès, and R. Barouki (1999)
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Regulation of cytosolic free calcium concentration by extracellular nucleotides in human hepatocytes.
C. Schofl, M. Ponczek, T. Mader, M. Waring, H. Benecke, A. von zur Muhlen, H. Mix, M. Cornberg, K. H. W. Boker, M. P. Manns, et al. (1999)
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Inhibition of Human Hepatitis B Virus Replication by AT-61, a Phenylpropenamide Derivative, Alone and in Combination with (-)beta -L-2',3'-Dideoxy-3'-Thiacytidine.
R. W. King, S. K. Ladner, T. J. Miller, K. Zaifert, R. B. Perni, S. C. Conway, and M. J. Otto (1998)
Antimicrob. Agents Chemother. 42, 3179-3186
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Orphan Receptor Hepatocyte Nuclear Factor-4 Antagonizes Estrogen Receptor {alpha}-Mediated Induction of Human Coagulation Factor XII Gene.
A. Farsetti, F. Moretti, M. Narducci, S. Misiti, S. Nanni, M. Andreoli, A. Sacchi, and A. Pontecorvi (1998)
Endocrinology 139, 4581-4589
   Abstract »    Full Text »    PDF »
Down-regulation of Cytochrome P450 1A1 Gene Promoter by Oxidative Stress. CRITICAL CONTRIBUTION OF NUCLEAR FACTOR 1.
Y. Morel and R. Barouki (1998)
J. Biol. Chem. 273, 26969-26976
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Fatty acid ethyl esters and HepG2 cells: intracellular synthesis and release from the cells.
A. Kabakibi, C. R. Morse, and M. Laposata (1998)
J. Lipid Res. 39, 1568-1582
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Regulation of the human p21/WAF1/Cip1 promoter in hepatic cells by functional interactions between Sp1 and Smad family members.
A. Moustakas and D. Kardassis (1998)
PNAS 95, 6733-6738
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Stabilization and cytoskeletal-association of LDL receptor mRNA are mediated by distinct domains in its 3' untranslated region.
G. M. Wilson, M. Z. Vasa, and R. G. Deeley (1998)
J. Lipid Res. 39, 1025-1032
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DNA methylation contributes to expression of the human neurotensin/neuromedin N gene.
Z. Dong, X. Wang, Q. Zhao, C. M. Townsend Jr., and B. M. Evers (1998)
Am J Physiol Gastrointest Liver Physiol 274, G535-G543
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A Novel nk-2-related Transcription Factor Associated with Human Fetal Liver and Hepatocellular Carcinoma.
G. A. Apergis, N. Crawford, D. Ghosh, C. M. Steppan, W. R. Vorachek, P. Wen, and J. Locker (1998)
J. Biol. Chem. 273, 2917-2925
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Role of Ceruloplasmin in Cellular Iron Uptake.
C. K. Mukhopadhyay, Z. K. Attieh, and P. L. Fox (1998)
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Cell Surface Glycoproteins Undergo Postbiosynthetic Modification of Their N-Glycans by Stepwise Demannosylation.
S. Porwoll, N. Loch, C. Kannicht, R. Nuck, D. Grunow, W. Reutter, and R. Tauber (1998)
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Interleukin-1ß and interleukin-6 increase levels of apolipoprotein B mRNA and decrease accumulation of its protein in culture medium of HepG2 cells.
K. Yokoyama, T. Ishibashi, L. Yi-qiang, A. Nagayoshi, T. Teramoto, and Y. Maruyama (1998)
J. Lipid Res. 39, 103-113
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Interleukin-6 Downregulates Factor XII Production by Human Hepatoma Cell Line (HepG2).
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A Functional Sp1 Binding Site Is Essential for the Activity of the Adult Liver-Specific Human Insulin-Like Growth Factor II Promoter.
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Contribution of a Nuclear Factor 1Binding Site to the Glucocorticoid Regulation of the Cytosolic Aspartate Aminotransferase Gene Promoter.
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The Hepatocyte Nuclear Factor 3beta Stimulates the Transcription of the Human Insulin-like Growth Factor I Gene in a Direct and Indirect Manner.
L. A. Nolten, P. H. Steenbergh, and J. S. Sussenbach (1996)
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The Role of beta gamma and alpha gamma Complexes in the Assembly of Human Fibrinogen.
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Two Separate Signal Transducer and Activator of Transcription Proteins Regulate Transcription of the Serine Proteinase Inhibitor-3 Gene in Hepatic Cells.
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Liver-enriched Transcription Factor HNF-4 and Ubiquitous Factor NF-Y Are Critical for Expression of Blood Coagulation Factor X.
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Functional Characterization of the Human Factor VII 5`-Flanking Region.
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Orphan Nuclear Receptor HNF-4 Binds to the Human Coagulation Factor VII Promoter.
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The Action of Interleukin-2 Receptor Subunits Defines a New Type of Signaling Mechanism for Hematopoietin Receptors in Hepatic Cells and Fibroblasts.
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The Promoter of the Salmon Insulin-like Growth Factor I Gene Is Activated by Hepatocyte Nuclear Factor 1.
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H. J. Mardon and G. Sebastio (1992)
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Growth requirements and expression of LDL receptor and HMG-CoA reductase in Hep G2 hepatoblastoma cells cultured in a chemically defined medium.
E. Gherardi, K. Thomas, T. D. Le Cras, C. Fitzsimmons, C. D. Moorby, and D. E. Bowyer (1992)
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Muscle cell components dictate hepatocyte gene expression and the distribution of the Golgi apparatus in heterokaryons..
S C Miller, G K Pavlath, B T Blakely, and H M Blau (1988)
Genes & Dev. 2, 330-340
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Estrogen memory effect in human hepatocytes during repeated cell division without hormone.
S. Tam, R. Hache, and R. Deeley (1986)
Science 234, 1234-1237
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Regulation of class III major histocompatibility complex gene products by interleukin-1.
D. Perlmutter, G Goldberger, C. Dinarello, S. Mizel, and H. Colten (1986)
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