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Science 10 August 1979:
Vol. 205. no. 4406, pp. 602 - 607
DOI: 10.1126/science.377496
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Articles

Science, Vol 205, Issue 4406, 602-607
Copyright © 1979 by American Association for the Advancement of Science

articles

Human growth hormone: complementary DNA cloning and expression in bacteria

JA Martial, RA Hallewell, JD Baxter, and HM Goodman

The nucleotide sequence of a DNA complementary to human growth hormone messenger RNA was cloned; it contains 29 nucleotides in its 5' untranslated region, the 651 nucleotides coding for the prehormone, and the entire 3' untranslated region (108 nucleotides). The data reported predict the previously unknown sequence of the signal peptide of human growth hormone and, by comparison with the previously determined sequences of rat growth hormone and human chorionic somatomammotropin, strengthens the hypothesis that these genes evolved by gene duplication from a common ancestral sequence. The human growth hormone gene sequences have been linked in phase to a fragment of the trp D gene of Escherichia coli in a plasmid vehicle, and a fusion protein is synthesized at high level (approximately 3 percent of bacterial protein) under the control of the regulatory region of the trp operon. This fusion protein (70 percent of whose amino acids are coded for by the human growth hormone gene) reacts specifically with antibodies to human growth hormone and is stable in E. coli.


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Hypomethylation of DNA from benign and malignant human colon neoplasms.
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The prolactin gene is located on chromosome 6 in humans.
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Comparison of the nucleic acid sequence of anglerfish and mammalian insulin mRNA's from cloned cDNA's.
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Silent nucleotide substitutions and the molecular evolutionary clock.
T. Jukes (1980)
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Genes for growth hormone, chorionic somatommammotropin, and growth hormones-like gene on chromosome 17 in humans.
D Owerbach, W. Rutter, J. Martial, J. Baxter, and T. Shows (1980)
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Growth Factors.
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Ann Intern Med 92, 650-662
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Chemical carcinogens: estimating the risk.
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