Reactome Array: Forging a Link Between Metabolome and Genome
Ana Beloqui,1,*
María-Eugenia Guazzaroni,1,*
Florencio Pazos,2
José M. Vieites,1
Marta Godoy,2
Olga V. Golyshina,3
Tatyana N. Chernikova,3
Agnes Waliczek,3
Rafael Silva-Rocha,2
Yamal Al-ramahi,1
Violetta La Cono,4
Carmen Mendez,5
José A. Salas,5
Roberto Solano,2
Michail M. Yakimov,4
Kenneth N. Timmis,3,6
Peter N. Golyshin,3,7,8,
,
Manuel Ferrer1,,
We describe a sensitive metabolite array for genome sequence–independent
functional analysis of metabolic phenotypes and networks, the
reactomes
, of cell populations and communities. The array includes
1676 dye-linked substrate compounds collectively representing
central metabolic pathways of all forms of life. Application
of cell extracts to the array leads to specific binding of enzymes
to cognate substrates, transformation to products, and concomitant
activation of the dye signals. Proof of principle was shown
by reconstruction of the metabolic maps of model bacteria. Utility
of the array for unsequenced organisms was demonstrated by reconstruction
of the global metabolisms of three microbial communities derived
from acidic volcanic pool, deep-sea brine lake, and hydrocarbon-polluted
seawater. Enzymes of interest are captured on nanoparticles
coated with cognate metabolites, sequenced, and their functions
unequivocally established.
2 CSIC, Centro Nacional de Biotecnología, 28049 Madrid, Spain.
3 HZI-Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.
4 Institute for Coastal Marine Environment, CNR, Messina 98122, Italy.
5 Universidad de Oviedo, 33006 Oviedo, Spain.
6 Institute of Microbiology, Carolo-Wilhelmina Technical University of Braunschweig, 38106 Braunschweig, Germany.
7 School of Biological Sciences, Bangor University, Gwynedd LL572 UW, UK.
8 Centre for Integrated Research in the Rural Environment, Aberystwyth University-Bangor University Partnership (CIRRE).
* These authors contributed equally to this work.
These authors contributed equally to this work.
To whom correspondence should be addressed. E-mail: mferrer{at}icp.csic.es (M.F.); p.golyshin{at}bangor.ac.uk (P.N.G.)
