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Science 18 September 2009:
Vol. 325. no. 5947, p. 1499
DOI: 10.1126/science.1176007
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Technical Comments

Response to Comment on "The Dynamic Control of Kiss-And-Run and Vesicular Reuse Probed with Single Nanoparticles"

Qi Zhang, Yulong Li, Richard W. Tsien*

Granseth et al. argue that vesicle retrieval at hippocampal synapses is fully accounted for by a single mode of endocytosis. However, their assay focused on readily releasable pool vesicles (RRP), not RRP + reserve pool vesicles in tandem, and therefore cannot detect pool-dependent changes in vesicle-retrieval kinetics during a stimulus train. Using a probe similar to theirs, we observed rapid vesicle retrieval consistent with Kiss-And-Run fusion.

Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA.

* To whom correspondence should be addressed. E-mail: rwtsien{at}stanford.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)