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Originally published in Science Express on 12 March 2009
Science 17 April 2009:
Vol. 324. no. 5925, pp. 392 - 397
DOI: 10.1126/science.1170540
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Reports

In Vivo Analysis of Dendritic Cell Development and Homeostasis

Kang Liu,1* Gabriel D. Victora,1{dagger} Tanja A. Schwickert,1{dagger} Pierre Guermonprez,1 Matthew M. Meredith,1 Kaihui Yao,1 Fei-Fan Chu,1 Gwendalyn J. Randolph,2 Alexander Y. Rudensky,3,4 Michel Nussenzweig1,4*

Dendritic cells (DCs) in lymphoid tissue arise from precursors that also produce monocytes and plasmacytoid DCs (pDCs). Where DC and monocyte lineage commitment occurs and the nature of the DC precursor that migrates from the bone marrow to peripheral lymphoid organs are unknown. We show that DC development progresses from the macrophage and DC precursor to common DC precursors that give rise to pDCs and classical spleen DCs (cDCs), but not monocytes, and finally to committed precursors of cDCs (pre-cDCs). Pre-cDCs enter lymph nodes through and migrate along high endothelial venules and later disperse and integrate into the DC network. Further cDC development involves cell division, which is controlled in part by regulatory T cells and fms-like tyrosine kinase receptor-3.

1 Laboratory of Molecular Immunology, Rockefeller University, New York, NY 10065, USA.
2 Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
3 Department of Immunology and Howard Hughes Medical Institute (HHMI), University of Washington, Seattle, WA 98195, USA.
4 HHMI, Rockefeller University, New York, NY 10021, USA.

{dagger} These authors contributed equally to this work.

* To whom correspondence should be addressed. E-mail: liuk{at}rockefeller.edu (L.K.); nussen{at}rockefeller.edu (M.N.)

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