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Originally published in Science Express on 12 February 2009
Science 13 March 2009:
Vol. 323. no. 5920, pp. 1448 - 1453
DOI: 10.1126/science.1167373
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Research Articles

The Dynamic Control of Kiss-And-Run and Vesicular Reuse Probed with Single Nanoparticles

Qi Zhang, Yulong Li, Richard W. Tsien*

Vesicular secretion of neurotransmitter is essential for neuronal communication. Kiss-and-run is a mode of membrane fusion and retrieval without the full collapse of the vesicle into the plasma membrane and de novo regeneration. The importance of kiss-and-run during efficient neurotransmission has remained in doubt. We developed an approach for loading individual synaptic vesicles with single quantum dots. Their size and pH-dependent photoluminescence change allowed us to distinguish kiss-and-run from full-collapse fusion and to track single vesicles through multiple rounds of kiss-and-run and reuse, without perturbing vesicle cycling. Kiss-and-run dominated at the beginning of stimulus trains, reflecting the preference of vesicles with high release probability. Its incidence was increased by rapid firing, a response appropriate to shape the kinetics of neurotransmission during a wide range of firing patterns.

Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA.

* To whom correspondence should be addressed. E-mail: rwtsien{at}stanford.edu

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