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Originally published in Science Express on 23 October 2008
Science 12 December 2008:
Vol. 322. no. 5908, pp. 1713 - 1717
DOI: 10.1126/science.1165216
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Reports

De Novo Formation of a Subnuclear Body

Trish E. Kaiser,1 Robert V. Intine,1,2 Miroslav Dundr1*

The mammalian cell nucleus contains structurally stable functional compartments. We show here that one of them, the Cajal body (CB), can be formed de novo. Immobilization on chromatin of both CB structural components, such as coilin, and functional components of the CB, such as the SMN complex, spliceosomal small nuclear ribonucleoproteins (RNPs), small nucleolar RNPs, and small Cajal body–specific RNPs, is sufficient for the formation of a morphologically normal and apparently functional CB. Biogenesis of the CB does not follow a hierarchical assembly pathway and exhibits hallmarks of a self-organizing structure.

1 Department of Cell Biology, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA.
2 Dr. William M. Scholl College of Podiatric Medicine at Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064, USA.

* To whom correspondence should be addressed. E-mail: mirek.dundr{at}rosalindfranklin.edu

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