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Originally published in Science Express on 7 August 2008
Science 5 September 2008:
Vol. 321. no. 5894, pp. 1354 - 1357
DOI: 10.1126/science.1161070
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Reports

Helical Structures of ESCRT-III Are Disassembled by VPS4

Suman Lata,1 Guy Schoehn,1,2 Ankur Jain,3* Ricardo Pires,1 Jacob Piehler,3 Heinrich G. Gottlinger,4 Winfried Weissenhorn1{dagger}

During intracellular membrane trafficking and remodeling, protein complexes known as the ESCRTs (endosomal sorting complexes required for transport) interact with membranes and are required for budding processes directed away from the cytosol, including the budding of intralumenal vesicles to form multivesicular bodies; for the budding of some enveloped viruses; and for daughter cell scission in cytokinesis. We found that the ESCRT-III proteins CHMP2A and CHMP3 (charged multivesicular body proteins 2A and 3) could assemble in vitro into helical tubular structures that expose their membrane interaction sites on the outside of the tubule, whereas the AAA-type adenosine triphosphatase VPS4 could bind on the inside of the tubule and disassemble the tubes upon adenosine triphosphate hydrolysis. CHMP2A and CHMP3 copolymerized in solution, and their membrane targeting was cooperatively enhanced on planar lipid bilayers. Such helical CHMP structures could thus assemble within the neck of an inwardly budding vesicle, catalyzing late steps in budding under the control of VPS4.

1 Unit for Virus Host Cell Interaction, UMR 5233 UJF (Université Joseph Fourier)-EMBL (European Molecular Biology Laboratory)-CNRS, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France.
2 Institut de Biologie Structurale UMR 5075 CEA (Comissariat à l'Énergie Atomique)-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France.
3 Institute of Biochemistry, Johann Wolfgang Goethe University, Max-von-Laue Straße 9, 60438 Frankfurt am Main, Germany.
4 Program in Gene Function and Expression, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.

* Present address: Department of Physics, University of Illinois at Urbana-Champaign, 1110 West Green Street, Urbana, IL 61801–3080, USA.

{dagger} To whom correspondence should be addressed. E-mail: weissenhorn{at}embl.fr

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