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Science 29 February 2008:
Vol. 319. no. 5867, pp. 1247 - 1250
DOI: 10.1126/science.1153634
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Reports

Membrane Proteins of the Endoplasmic Reticulum Induce High-Curvature Tubules

Junjie Hu,1 Yoko Shibata,1 Christiane Voss,2 Tom Shemesh,3 Zongli Li,4 Margaret Coughlin,5 Michael M. Kozlov,3 Tom A. Rapoport,1* William A. Prinz2*

The tubular structure of the endoplasmic reticulum (ER) appears to be generated by integral membrane proteins, the reticulons and a protein family consisting of DP1 in mammals and Yop1p in yeast. Here, individual members of these families were found to be sufficient to generate membrane tubules. When we purified yeast Yop1p and incorporated it into proteoliposomes, narrow tubules (~15 to 17 nanometers in diameter) were generated. Tubule formation occurred with different lipids; required essentially only the central portion of the protein, including its two long hydrophobic segments; and was prevented by mutations that affected tubule formation in vivo. Tubules were also formed by reconstituted purified yeast Rtn1p. Tubules made in vitro were narrower than normal ER tubules, due to a higher concentration of tubule-inducing proteins. The shape and oligomerization of the "morphogenic" proteins could explain the formation of the tubular ER.

1 Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
2 Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Disorders (NIDDK), National Institutes of Health, Bethesda, MD 20892, USA.
3 Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, 69978 Tel Aviv, Israel.
4 Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
5 Department of Systems Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.

* To whom correspondence should be addressed. E-mail: tom_rapoport{at}hms.harvard.edu (T.A.R.); wprinz{at}helix.nih.gov (W.A.P.)

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Reticulon and Dp1/Yop1p Proteins Form Immobile Oligomers in the Tubular Endoplasmic Reticulum.
Y. Shibata, C. Voss, J. M. Rist, J. Hu, T. A. Rapoport, W. A. Prinz, and G. K. Voeltz (2008)
J. Biol. Chem. 283, 18892-18904
   Abstract »    Full Text »    PDF »
How the ER gets its shape.
R. Robinson (2008)
J. Cell Biol. 180, 1055
   Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)