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Science 25 January 2008:
Vol. 319. no. 5862, pp. 482 - 484
DOI: 10.1126/science.1151582
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Reports

The Frequency Dependence of Osmo-Adaptation in Saccharomyces cerevisiae

Jerome T. Mettetal,1 Dale Muzzey,1,2 Carlos Gómez-Uribe,1,3 Alexander van Oudenaarden1*

The propagation of information through signaling cascades spans a wide range of time scales, including the rapid ligand-receptor interaction and the much slower response of downstream gene expression. To determine which dynamic range dominates a response, we used periodic stimuli to measure the frequency dependence of signal transduction in the osmo-adaptation pathway of Saccharomyces cerevisiae. We applied system identification methods to infer a concise predictive model. We found that the dynamics of the osmo-adaptation response are dominated by a fast-acting negative feedback through the kinase Hog1 that does not require protein synthesis. After large osmotic shocks, an additional, much slower, negative feedback through gene expression allows cells to respond faster to future stimuli.

1 Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
2 Harvard University Graduate Biophysics Program, Harvard Medical School, Boston, MA 02115, USA.
3 Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

* To whom correspondence should be addressed: E-mail: avano{at}mit.edu

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Stress resistance and signal fidelity independent of nuclear MAPK function.
P. J. Westfall, J. C. Patterson, R. E. Chen, and J. Thorner (2008)
PNAS 105, 12212-12217
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Science. ISSN 0036-8075 (print), 1095-9203 (online)