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Science 18 January 2008:
Vol. 319. no. 5861, p. 294
DOI: 10.1126/science.1150083
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Brevia

Arabidopsis CLV3 Peptide Directly Binds CLV1 Ectodomain

Mari Ogawa, Hidefumi Shinohara, Youji Sakagami, Yoshikatsu Matsubayashi*

CLV1, which encodes a leucine-rich repeat receptor kinase, and CLV3, which encodes a secreted peptide, function in the same genetic pathway to maintain stem cell populations in Arabidopsis shoot apical meristem. Here, we show biochemical evidence, by ligand binding assay and photoaffinity labeling, that the CLV3 peptide directly binds the CLV1 ectodomain with a dissociation constant of 17.5 nM. The CLV1 ectodomain also interacts with the structurally related CLE peptides, with distinct affinities depending on the specific amino acid sequence. Our results provide direct evidence that CLV3 and CLV1 function as a ligand-receptor pair involved in stem cell maintenance.

Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.

* To whom correspondence should be addressed. E-mail: matsu{at}agr.nagoya-u.ac.jp

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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N. Geldner and S. Robatzek (2008)
Plant Physiology 147, 1565-1574
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The EPIP Peptide of INFLORESCENCE DEFICIENT IN ABSCISSION Is Sufficient to Induce Abscission in Arabidopsis through the Receptor-Like Kinases HAESA and HAESA-LIKE2.
G.-E. Stenvik, N. M. Tandstad, Y. Guo, C.-L. Shi, W. Kristiansen, A. Holmgren, S. E. Clark, R. B. Aalen, and M. A. Butenko (2008)
PLANT CELL 20, 1805-1817
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A Genome-Wide Functional Investigation into the Roles of Receptor-Like Proteins in Arabidopsis.
G. Wang, U. Ellendorff, B. Kemp, J. W. Mansfield, A. Forsyth, K. Mitchell, K. Bastas, C.-M. Liu, A. Woods-Tor, C. Zipfel, et al. (2008)
Plant Physiology 147, 503-517
   Abstract »    Full Text »    PDF »
The Receptor Kinase CORYNE of Arabidopsis Transmits the Stem Cell-Limiting Signal CLAVATA3 Independently of CLAVATA1.
R. Muller, A. Bleckmann, and R. Simon (2008)
PLANT CELL 20, 934-946
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Science. ISSN 0036-8075 (print), 1095-9203 (online)