Response to Comment on "Protein Sequences from Mastodon and Tyrannosaurus rex Revealed by Mass Spectrometry"

doi:10.1126/science.1147364

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Science 4 January 2008:
Vol. 319. no. 5859, p. 33
DOI: 10.1126/science.1147364

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Technical Comments

Response to Comment on "Protein Sequences from Mastodon and Tyrannosaurus rex Revealed by Mass Spectrometry"

John M. Asara¹^,2^* and Mary H. Schweitzer³^,4

We sequenced six endogenous collagen peptides from Tyrannosaurusrex bone fragments using mass spectrometry. Five sequences matchbirds, but only two match amphibians, supporting dinosaur-birdrelationships. Buckley et al. reinterpret and misinterpret ourdata and question sequence authenticity, but they used a suboptimalphylogenetic algorithm to analyze only a subset of reportedsequences and they suggest analyses that are less sensitiveand less specific than mass spectrometry. We disagree and usedata to explain.

¹ Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.
² Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
³ Department of Marine, Earth, and Atmospheric Sciences, North Carolina State University, Raleigh, NC 27695, USA.
⁴ North Carolina Museum of Natural Sciences, Raleigh, NC 27601, USA.

^* To whom correspondence should be addressed. E-mail: jasara{at}bidmc.harvard.edu

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TECHNICAL COMMENTS Comment on "Protein Sequences from Mastodon and Tyrannosaurus rex Revealed by Mass Spectrometry": Mike Buckley, Angela Walker, Simon Y. W. Ho, Yue Yang, Colin Smith, Peter Ashton, Jane Thomas Oates, Enrico Cappellini, Hannah Koon, Kirsty Penkman, Ben Elsworth, Dave Ashford, Caroline Solazzo, Phillip Andrews, John Strahler, Beth Shapiro, Peggy Ostrom, Hasand Gandhi, Webb Miller, Brian Raney, Maria Ines Zylber, M. Thomas P. Gilbert, Richard V. Prigodich, Michael Ryan, Kenneth F. Rijsdijk, Anwar Janoo, and Matthew J. Collins (4 January 2008)
Science 319 (5859), 33c. [DOI: 10.1126/science.1147046]
| Abstract » | Full Text » | PDF » | Supporting Online Material »

REPORTS Protein Sequences from Mastodon and Tyrannosaurus Rex Revealed by Mass Spectrometry: John M. Asara, Mary H. Schweitzer, Lisa M. Freimark, Matthew Phillips, and Lewis C. Cantley (13 April 2007)
Science 316 (5822), 280. [DOI: 10.1126/science.1137614]
| Abstract » | Full Text » | PDF » | Supporting Online Material »

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:

Response to Comment on "Protein Sequences from Mastodon and Tyrannosaurus rex Revealed by Mass Spectrometry".: J. M. Asara, M. H. Schweitzer, L. C. Cantley, and J. S. Cottrell (2008)
Science 321, 1040c
| Abstract » | Full Text » | PDF »

Molecular Phylogenetics of Mastodon and Tyrannosaurus rex.: C. L. Organ, M. H. Schweitzer, W. Zheng, L. M. Freimark, L. C. Cantley, and J. M. Asara (2008)
Science 320, 499
| Abstract » | Full Text » | PDF »