Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 21 December 2007:
Vol. 318. no. 5858, p. 1888
DOI: 10.1126/science.1149751
Prev | Table of Contents | Next

Brevia

Combinatorial Synthesis of Peptide Arrays onto a Microchip

Mario Beyer,1* Alexander Nesterov,1* Ines Block,1 Kai König,1 Thomas Felgenhauer,1 Simon Fernandez,1 Klaus Leibe,1 Gloria Torralba,2 Michael Hausmann,2 Ulrich Trunk,2 Volker Lindenstruth,2{dagger} F. Ralf Bischoff,1{dagger}{ddagger} Volker Stadler,1{dagger}{ddagger} Frank Breitling1{dagger}{ddagger}

Arrays promise to advance biology through parallel screening for binding partners. We show the combinatorial in situ synthesis of 40,000 peptide spots per square centimeter on a microchip. Our variant Merrifield synthesis immobilizes activated amino acids as monomers within particles, which are successively attracted by electric fields generated on each pixel electrode of the chip. With all different amino acids addressed, particles are melted at once to initiate coupling. Repetitive coupling cycles should allow for the translation of whole proteomes into arrays of overlapping peptides that could be used for proteome research and antibody profiling.

1 German Cancer Research Center, INF 580, 69120 Heidelberg, Germany.
2 Kirchhoff Institute for Physics, University of Heidelberg, INF 227, 69120 Heidelberg, Germany.

* These authors contributed equally to this work.

{ddagger} These authors contributed equally to this work.

{dagger} To whom correspondence should be addressed. E-mail: f.breitling{at}dkfz.de (F.B.); r.bischoff{at}dkfz.de (F.R.B.); v.stadler{at}dkfz.de (V.S.); voli{at}kip.uni-heidelberg.de (V.L.)

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Photoelectrochemical synthesis of DNA microarrays.
B. Y. Chow, C. J. Emig, and J. M. Jacobson (2009)
PNAS 106, 15219-15224
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)