Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 1 November 2007
Science 7 December 2007:
Vol. 318. no. 5856, pp. 1640 - 1642
DOI: 10.1126/science.1146453
Prev | Table of Contents | Next

Reports

Engineering Modified Bt Toxins to Counter Insect Resistance

Mario Soberón,1* Liliana Pardo-López,1 Idalia López,1 Isabel Gómez,1 Bruce E. Tabashnik,2 Alejandra Bravo1*

The evolution of insect resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxins that are widely used in sprays and transgenic crops. Resistance to Bt toxins in some insects is linked with mutations that disrupt a toxin-binding cadherin protein. We show that susceptibility to the Bt toxin Cry1Ab was reduced by cadherin gene silencing with RNA interference in Manduca sexta, confirming cadherin's role in Bt toxicity. Native Cry1A toxins required cadherin to form oligomers, but modified Cry1A toxins lacking one {alpha}-helix did not. The modified toxins killed cadherin-silenced M. sexta and Bt-resistant Pectinophora gossypiella that had cadherin deletion mutations. Our findings suggest that cadherin promotes Bt toxicity by facilitating toxin oligomerization and demonstrate that the modified Bt toxins may be useful against pests resistant to standard Bt toxins.

1 Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca 62250, Morelos, Mexico.
2 Department of Entomology, University of Arizona, Tucson, AZ, USA.

* To whom correspondence should be addressed. E-mail: bravo{at}ibt.unam.mx (A.B.); mario{at}ibt.unam.mx (M.S.)

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Outlook for Protein Engineering in Crop Improvement.
A. G. Rao (2008)
Plant Physiology 147, 6-12
   Full Text »    PDF »
Bacillus thuringiensis Cry1Ac Toxin-Binding and Pore-Forming Activity in Brush Border Membrane Vesicles Prepared from Anterior and Posterior Midgut Regions of Lepidopteran Larvae.
A. Rodrigo-Simon, S. Caccia, and J. Ferre (2008)
Appl. Envir. Microbiol. 74, 1710-1716
   Abstract »    Full Text »    PDF »
Biotechnological Prospects for Engineering Insect-Resistant Plants.
J. A. Gatehouse (2008)
Plant Physiology 146, 881-887
   Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)