Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
TaqMan Express Plates

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 13 September 2007
Science 12 October 2007:
Vol. 318. no. 5848, pp. 261 - 265
DOI: 10.1126/science.1146994
Prev | Table of Contents | Next

Reports

FKF1 and GIGANTEA Complex Formation Is Required for Day-Length Measurement in Arabidopsis

Mariko Sawa, Dmitri A. Nusinow, Steve A. Kay, Takato Imaizumi*

Precise timing of CONSTANS (CO) gene expression is necessary for day-length discrimination for photoperiodic flowering. The FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1), and GIGANTEA (GI) proteins regulate CO transcription in Arabidopsis. We demonstrate that FKF1 and GI proteins form a complex in a blue-light–dependent manner. The timing of this interaction regulates the timing of daytime CO expression. FKF1 function is dependent on GI, which interacts with a CO repressor, CYCLING DOF FACTOR 1 (CDF1), and controls CDF1 stability. GI, FKF1, and CDF1 proteins associate with CO chromatin. Thus, the FKF1-GI complex forms on the CO promoter in late afternoon to regulate CO expression, providing a mechanistic view of how the coincidence of light with circadian timing regulates photoperiodic flowering.

Department of Biochemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.

Present address: Division of Biological Sciences, University of California, San Diego, 9800 Gilman Drive #0130, La Jolla, CA 92093–0116, USA.

* To whom correspondence should be addressed. E-mail: timaizumi{at}ucsd.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Suppression of Pleiotropic Effects of Functional CRYPTOCHROME Genes by TERMINAL FLOWER 1.
A. S. Buchovsky, B. Strasser, P. D. Cerdan, and J. J. Casal (2008)
Genetics 180, 1467-1474
   Abstract »    Full Text »    PDF »
Acceleration of Flowering during Shade Avoidance in Arabidopsis Alters the Balance between FLOWERING LOCUS C-Mediated Repression and Photoperiodic Induction of Flowering.
A. C. Wollenberg, B. Strasser, P. D. Cerdan, and R. M. Amasino (2008)
Plant Physiology 148, 1681-1694
   Abstract »    Full Text »    PDF »
Ehd2, a Rice Ortholog of the Maize INDETERMINATE1 Gene, Promotes Flowering by Up-Regulating Ehd1.
K. Matsubara, U. Yamanouchi, Z.-X. Wang, Y. Minobe, T. Izawa, and M. Yano (2008)
Plant Physiology 148, 1425-1435
   Abstract »    Full Text »    PDF »
Two New Clock Proteins, LWD1 and LWD2, Regulate Arabidopsis Photoperiodic Flowering.
J.-F. Wu, Y. Wang, and S.-H. Wu (2008)
Plant Physiology 148, 948-959
   Abstract »    Full Text »    PDF »
Flowering Newsletter bibliography for 2007.
Compiled by, F. Tooke, T. Chiurugwi, and N. Battey (2008)
J. Exp. Bot.
   Full Text »    PDF »
Disruption of the Arabidopsis Circadian Clock Is Responsible for Extensive Variation in the Cold-Responsive Transcriptome.
Z. Bieniawska, C. Espinoza, A. Schlereth, R. Sulpice, D. K. Hincha, and M. A. Hannah (2008)
Plant Physiology 147, 263-279
   Abstract »    Full Text »    PDF »
Effects of Blue Light Deficiency on Acclimation of Light Energy Partitioning in PSII and CO2 Assimilation Capacity to High Irradiance in Spinach Leaves.
R. Matsuda, K. Ohashi-Kaneko, K. Fujiwara, and K. Kurata (2008)
Plant Cell Physiol. 49, 664-670
   Abstract »    Full Text »    PDF »
Identification of Dynamin as an Interactor of Rice GIGANTEA by Tandem Affinity Purification (TAP).
M. Abe, M. Fujiwara, K.-i. Kurotani, S. Yokoi, and K. Shimamoto (2008)
Plant Cell Physiol. 49, 420-432
   Abstract »    Full Text »    PDF »
Insight into Missing Genetic Links Between Two Evening-Expressed Pseudo-Response Regulator Genes TOC1 and PRR5 in the Circadian Clock-Controlled Circuitry in Arabidopsis thaliana.
S. Ito, Y. Niwa, N. Nakamichi, H. Kawamura, T. Yamashino, and T. Mizuno (2008)
Plant Cell Physiol. 49, 201-213
   Abstract »    Full Text »    PDF »
COP1-Mediated Ubiquitination of CONSTANS Is Implicated in Cryptochrome Regulation of Flowering in Arabidopsis.
L.-J. Liu, Y.-C. Zhang, Q.-H. Li, Y. Sang, J. Mao, H.-L. Lian, L. Wang, and H.-Q. Yang (2008)
PLANT CELL 20, 292-306
   Abstract »    Full Text »    PDF »
In Vivo Phosphorylation Site Mapping and Functional Characterization of Arabidopsis Phototropin 1.
S. Sullivan, C. E. Thomson, D. J. Lamont, M. A. Jones, and J. M. Christie (2008)
Mol Plant 1, 178-194
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)