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Quantitative Mass Spectrometry Identifies Insulin Signaling Targets in C. elegans
Meng-Qiu Dong,1John D. Venable,1Nora Au,2,3Tao Xu,1Sung Kyu Park,1Daniel Cociorva,1Jeffrey R. Johnson,1Andrew Dillin,2John R. Yates, III1*
DAF-2, an insulin receptor–like protein, regulates metabolism,development, and aging in Caenorhabditis elegans. In a quantitativeproteomic study, we identified 86 proteins that were more orless abundant in long-lived daf-2 mutant worms than in wild-typeworms. Genetic studies on a subset of these proteins indicatedthat they act in one or more processes regulated by DAF-2, includingentry into the dauer developmental stage and aging. In particular,we discovered a compensatory mechanism activated in responseto reduced DAF-2 signaling, which involves the protein phosphatasecalcineurin.
1 Scripps Research Institute, La Jolla, CA92037, USA. 2 Salk Institute for Biological Studies, La Jolla, CA 92037, USA. 3 University of California at San Diego, La Jolla, CA 92093, USA.
* To whom correspondence should be addressed. E-mail: jyates{at}scripps.edu
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