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Originally published in Science Express on 7 June 2007
Science 29 June 2007:
Vol. 316. no. 5833, pp. 1908 - 1912
DOI: 10.1126/science.1143422
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Reports

Parallels Between Cytokinesis and Retroviral Budding: A Role for the ESCRT Machinery

Jez G. Carlton and Juan Martin-Serrano*

During cytokinesis, as dividing animal cells pull apart into two daughter cells, the final stage, termed abscission, requires breakage of the midbody, a thin membranous stalk connecting the daughter cells. This membrane fission event topologically resembles the budding of viruses, such as HIV-1, from infected cells. We found that two proteins involved in HIV-1 budding—tumor susceptibility gene 101 (Tsg101), a subunit of the endosomal sorting complex required for transport I (ESCRT-I), and Alix, an ESCRT-associated protein—were recruited to the midbody during cytokinesis by interaction with centrosome protein 55 (Cep55), a centrosome and midbody protein essential for abscission. Tsg101, Alix, and possibly other components of ESCRT-I were required for the completion of cytokinesis. Thus, HIV-1 budding and cytokinesis use a similar subset of cellular components to carry out topologically similar membrane fission events.

Department of Infectious Diseases, King's College London School of Medicine, London, UK.

* To whom correspondence should be addressed. E-mail: juan.martin_serrano{at}kcl.ac.uk

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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NEDD4L Overexpression Rescues the Release and Infectivity of Human Immunodeficiency Virus Type 1 Constructs Lacking PTAP and YPXL Late Domains.
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A. Mukai, E. Mizuno, K. Kobayashi, M. Matsumoto, K. I. Nakayama, N. Kitamura, and M. Komada (2008)
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Human Immunodeficiency Virus Type 1 Gag Engages the Bro1 Domain of ALIX/AIP1 through the Nucleocapsid.
S. Popov, E. Popova, M. Inoue, and H. G. Gottlinger (2008)
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Regulation of Tsg101 Expression by the Steadiness Box: A Role of Tsg101-associated Ligase.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)